Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Acinetobacter baylyi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | weak inhibition | Acinetobacter baylyi | |
N-ethylmaleimide | - |
Acinetobacter baylyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
NADH | pH 7.4, 30°C | Acinetobacter baylyi | |
0.28 | - |
benzaldehyde | pH 7.4, 30°C | Acinetobacter baylyi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 0.01 mM, residual activity: 0.2% | Acinetobacter baylyi | |
Cu2+ | 0.1 mM, residual activity: 1.3% | Acinetobacter baylyi | |
Fe2+ | 1 mM, residual activity: 21.7% | Acinetobacter baylyi | |
Hg+ | 0.001 mM, residual activity: 0.1% | Acinetobacter baylyi | |
Mg2+ | 1 mM, activity enhanced to 128% | Acinetobacter baylyi | |
Mn2+ | 1 mM, activity enhanced to 128% | Acinetobacter baylyi | |
Zn2+ | 1 mM, residual activity: 1.2% | Acinetobacter baylyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39500 | - |
SDS-PAGE | Acinetobacter baylyi |
72600 | - |
gel filtration | Acinetobacter baylyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baylyi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ion-exchange chromatography, hydrophobic interaction chromatography, ultrafiltration, affinity chromatography | Acinetobacter baylyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
anisaldehyde + NADH + H+ | relative activity: 72.2% | Acinetobacter baylyi | anisic alcohol + NAD+ | - |
r | |
benzaldehyde + NADH + H+ | relative activity: 100% | Acinetobacter baylyi | benzyl alcohol + NAD+ | - |
r | |
cinnamaldehyde + NADH + H+ | relative activity: 58.7% | Acinetobacter baylyi | cinnamic alcohol + NAD+ | - |
r | |
cis-11-hexadecenal + NADH + H+ | relative activity: 4.9% | Acinetobacter baylyi | cis-11-hexadecenol + NAD+ | - |
r | |
citral + NADH + H+ | relative activity: 7.8% | Acinetobacter baylyi | citryl alcohol + NAD+ | - |
r | |
citronellal + NADH + H+ | relative activity: 21.8% | Acinetobacter baylyi | citronellol + NAD+ | - |
r | |
coniferyl alcohol + NAD+ | relative activity: 96% | Acinetobacter baylyi | coniferyl aldehyde + NADH + H+ | - |
r | |
decanal + NADH + H+ | relative activity: 19.7% | Acinetobacter baylyi | decanol + NAD+ | - |
r | |
dodecanal + NADH + H+ | relative activity: 8.9% | Acinetobacter baylyi | dodecanol + NAD+ | - |
r | |
geranial + NADH + H+ | - |
Acinetobacter baylyi | geraniol + NAD+ | - |
r | |
glutaraldehyde + NADH + H+ | relative activity: 0.6% | Acinetobacter baylyi | ? + NAD+ | - |
r | |
glyoxal + NADH + H+ | relative activity: 0.7% | Acinetobacter baylyi | ? + NAD+ | - |
r | |
hexanal + NADH + H+ | relative activity: 6.4% | Acinetobacter baylyi | hexanol + NAD+ | - |
r | |
octanal + NADH + H+ | relative activity: 36.5% | Acinetobacter baylyi | octanol + NAD+ | - |
r | |
salicyl aldehyde + NADH + H+ | relative activity: 9% | Acinetobacter baylyi | salicyl alcohol + NAD+ | - |
r | |
vanillin + NADH + H+ | relative activity: 4% | Acinetobacter baylyi | vanillin alcohol + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39500, gel filtration | Acinetobacter baylyi |
Synonyms | Comment | Organism |
---|---|---|
ADP1 | - |
Acinetobacter baylyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Acinetobacter baylyi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
enzyme reaches 52% of its maximum activity at 15°C | Acinetobacter baylyi |
70 | - |
enzyme loses 94% of its activity after 2 min of incubation at 70°C | Acinetobacter baylyi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8920 | - |
NADH | pH 7.4, 30°C | Acinetobacter baylyi | |
12940 | - |
benzaldehyde | pH 7.4, 30°C | Acinetobacter baylyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Acinetobacter baylyi |
General Information | Comment | Organism |
---|---|---|
malfunction | disruption mutant shows similar growth compared to wild-type | Acinetobacter baylyi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
46700 | - |
benzaldehyde | pH 7.4, 30°C | Acinetobacter baylyi | |
528000 | - |
NADH | pH 7.4, 30°C | Acinetobacter baylyi |