Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | isoenzyme 1, 1 mM, 19% inhibition | Saccharomyces cerevisiae | |
cysteine | isoenzyme 1, 1 mM, 29% inhibition | Saccharomyces cerevisiae | |
dithiothreitol | isoenzyme 1, 1 mM, 41% inhibition, 10 mM, 87% inhibition | Saccharomyces cerevisiae | |
glutathione | isoenzyme 1, 1 mM, 12% inhibition | Saccharomyces cerevisiae | |
iodoacetamide | isoenzyme 2, 1 mM, 87% inhibition, isoenzyme 1, 1 mM, 17% inhibition | Saccharomyces cerevisiae | |
iodoacetate | isoenzyme 2, 1 mM, 97% inhibition, isoenzyme 1, 1 mM, 18% inhibition | Saccharomyces cerevisiae | |
nitrate | isoenzyme 1, 50 mM, 50% inhibition | Saccharomyces cerevisiae | |
p-chloromercuribenzoate | isoenzyme 2, 1 mM, complete inhibition, 0.1 mM, 88% inhibition, 29% inhibition in the presence of 1 mM dithiothreitol, 21% inhibition in the presence of 1 mM L-cysteine, isoenzyme 1, 1 mM, 59% inhibition | Saccharomyces cerevisiae | |
Sodium fluoride | isoenzyme 1, 10 mM, 32% inhibition | Saccharomyces cerevisiae | |
sodium iodide | isoenzyme 1, 50 mM, 37% inhibition of glyoxylate reduction | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NADPH | isoenzyme 1 | Saccharomyces cerevisiae | |
13 | - |
glyoxylate | isoenzyme 1 | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
isoenzyme 1, gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NAD(P)H | Saccharomyces cerevisiae | - |
glycolate + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isoenzyme 1, protamine sulfate, DEAE-cellulose, hydroxylapatite, Sephadex G-150, DEAE-cellulose, hydroxylapatite | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.89 | - |
isoenzyme 1 | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
isoenzyme 1, 4°C, 10 mM potassium phosphate, pH 7.0, 1 week, 20% loss of activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxal + NADPH | isoenzyme 1, 16% activity of glyxoxylate | Saccharomyces cerevisiae | glycol + NADP+ | - |
? | |
glyoxylate + NAD(P)H | - |
Saccharomyces cerevisiae | glycolate + NAD(P)+ | - |
? | |
glyoxylate + NAD(P)H | - |
Saccharomyces cerevisiae | glycolate + NAD(P)+ | - |
ir | |
hydroxypyruvate + NAD(P)H + H+ | isoenzyme 1, hydroxypyruvate shows 15% of the activity of glyoxylate | Saccharomyces cerevisiae | glycerate + NAD(P)+ | - |
? | |
oxaloacetate + NADPH | isoenzyme 1, 12% activity of glyoxylate | Saccharomyces cerevisiae | malate + NADP+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
isoenzyme 1, 20% loss of activity after 5 min, complete inactivation above 50°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.2 | isoenzyme 1 | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | specific for NADPH | Saccharomyces cerevisiae | |
NADPH | specific for NADPH | Saccharomyces cerevisiae |