KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
NADPH | - |
Spinacia oleracea | |
0.085 | - |
glyoxylate | cofactor NADPH | Spinacia oleracea | |
0.15 | - |
NADH | - |
Spinacia oleracea | |
1.1 | - |
glyoxylate | cofactor NADH | Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
4 * 33000, SDS-PAGE | Spinacia oleracea |
125000 | - |
gel filtration | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NAD(P)H | Triticum aestivum | - |
glycolate + NAD(P)+ | - |
? | |
glyoxylate + NAD(P)H | Spinacia oleracea | - |
glycolate + NAD(P)+ | - |
? | |
glyoxylate + NAD(P)H | Glycine max | - |
glycolate + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Spinacia oleracea | - |
- |
- |
Triticum aestivum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, affinity chromatography, Sephadex G-75SF, AgdApP-4 | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.45 | 0.7 | glyoxylate reduction in crude extracts | Triticum aestivum |
0.45 | 0.7 | glyoxylate reduction in crude extracts | Glycine max |
210.6 | - |
- |
Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NAD(P)H | - |
Triticum aestivum | glycolate + NAD(P)+ | - |
? | |
glyoxylate + NAD(P)H | - |
Spinacia oleracea | glycolate + NAD(P)+ | - |
? | |
glyoxylate + NAD(P)H | - |
Glycine max | glycolate + NAD(P)+ | - |
? | |
glyoxylate + NADH + H+ | - |
Spinacia oleracea | glycolate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33000, SDS-PAGE | Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.4 | - |
Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Triticum aestivum | |
NAD(P)H | - |
Spinacia oleracea | |
NAD(P)H | - |
Glycine max |