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Literature summary for 1.1.1.67 extracted from

  • Jang, M.; Park, J.; Kim, M.; Lee, S.; Kang, J.; Kim, T.
    Molecular cloning and gene expression of Sinorhizobium meliloti mannitol dehydrogenase in Escherichia coli, and its enzymatic characterization (2013), Korean J. Microbiol. Biotechnol., 41, 153-159.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Sinorhizobium meliloti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
x * 54000, calculated from amino acid sequence Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose + NADH + H+ Sinorhizobium meliloti
-
D-mannitol + NAD+
-
r
D-fructose + NADH + H+ Sinorhizobium meliloti KCTC 2353
-
D-mannitol + NAD+
-
r
D-mannitol + NAD+ Sinorhizobium meliloti
-
D-fructose + NADH + H+
-
r
D-mannitol + NAD+ Sinorhizobium meliloti KCTC 2353
-
D-fructose + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti
-
-
-
Sinorhizobium meliloti KCTC 2353
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + NADH + H+
-
Sinorhizobium meliloti D-mannitol + NAD+
-
r
D-fructose + NADH + H+
-
Sinorhizobium meliloti KCTC 2353 D-mannitol + NAD+
-
r
D-mannitol + NAD+
-
Sinorhizobium meliloti D-fructose + NADH + H+
-
r
D-mannitol + NAD+
-
Sinorhizobium meliloti KCTC 2353 D-fructose + NADH + H+
-
r
additional information no activity with NADP+ and NADPH Sinorhizobium meliloti ?
-
?
additional information no activity with NADP+ and NADPH Sinorhizobium meliloti KCTC 2353 ?
-
?

Subunits

Subunits Comment Organism
? x * 54000, calculated from amino acid sequence Sinorhizobium meliloti

Synonyms

Synonyms Comment Organism
MDH
-
Sinorhizobium meliloti
NAD+-dependent mannitol dehydrogenase
-
Sinorhizobium meliloti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
optimum pH for D-fructose reduction Sinorhizobium meliloti
10
-
optimum pH for D-mannitol oxidation Sinorhizobium meliloti

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Sinorhizobium meliloti