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Literature summary for 1.1.1.67 extracted from

  • Baeumchen, C.; Roth, A.H.; Biedendieck, R.; Malten, M.; Follmann, M.; Sahm, H.; Bringer-Meyer, S.; Jahn, D.
    D-mannitol production by resting state whole cell biotransformation of D-fructose by heterologous mannitol and formate dehydrogenase gene expression in Bacillus megaterium (2007), Biotechnol. J., 2, 1408-1416.
    View publication on PubMed
Search for more literature for 1.1.1.67

Application

Application Comment Organism
synthesis the recombinant enzyme expressed in Bacillus megaterium is useful in production of D-mannitol using a resting cell biotransformation approach Leuconostoc pseudomesenteroides

Cloned(Commentary)

Cloned (Comment) Organism
gene mdh, high level expression in Bacillus megaterium requiring the adaptation of the corresponding ribosome binding site, the fdh gene is adapted to Bacillus megaterium codon usage via complete chemical gene synthesis, overview Leuconostoc pseudomesenteroides

Protein Variants

Protein Variants Comment Organism
additional information D-mannitol production by resting state whole cell biotransformation of D-fructose by heterologous mannitol dehydrogenase gene from Leuconostoc pseudomesenteroides and the formate dehydrogenase gene, gene fdh from Mycobacterium vaccae N10, expression in Bacillus megaterium, development of an in vivo system, overview Leuconostoc pseudomesenteroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose + NADH + H+ Leuconostoc pseudomesenteroides -mannitol production D-mannitol + NAD+
-
 r
D-fructose + NADH + H+ Leuconostoc pseudomesenteroides ATCC 12291 -mannitol production D-mannitol + NAD+
-
 r

Organism

Organism UniProt Comment Textmining
Leuconostoc pseudomesenteroides Q83VI5 strain ATCC12291, gene mdh
-
Leuconostoc pseudomesenteroides ATCC 12291 Q83VI5 strain ATCC12291, gene mdh
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + NADH + H+
-
 Leuconostoc pseudomesenteroides D-mannitol + NAD+
-
 r
D-fructose + NADH + H+ -mannitol production Leuconostoc pseudomesenteroides D-mannitol + NAD+
-
 r
D-fructose + NADH + H+
-
 Leuconostoc pseudomesenteroides ATCC 12291 D-mannitol + NAD+
-
 r
D-fructose + NADH + H+ -mannitol production Leuconostoc pseudomesenteroides ATCC 12291 D-mannitol + NAD+
-
 r