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Literature summary for 1.1.1.67 extracted from

  • Klimacek, M.; Kavanagh, K.L.; Wilson, D.K.; Nidetzky, B.
    On the role of Bronsted catalysis in Pseudomonas fluorescens mannitol 2-dehydrogenase (2003), Biochem. J., 375, 141-149.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
K295A 30000fold lower turnover number for D-mannitol oxidation at pH 10.0 than the wild-type enzyme Pseudomonas fluorescens
K295M 2000000fold lower turnover number for D-mannitol oxidation at pH 10.0 than the wild-type enzyme Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannitol + NAD+ the epsilon-NH2 group of Lys295 participates in an obligatory pH-dependent, pre-catalytic equilibrium which may control alcohol/alkoxide equilibration of the enzyme-bound D-mannitol and activates the C2 atom for subsequent catalytic oxidation by NAD+ Pseudomonas fluorescens D-fructose + NADH + H+
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