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Literature summary for 1.1.1.67 extracted from
- Crystalline D-mannitol:NAD oxidoreductase from Leuconostoc mesenteroides: Part II. substrate and coenzyme specificity (1968), Agric. Biol. Chem., 32, 894-899.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Leuconostoc mesenteroides |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | 0.1 mM, 100% inhibition | Leuconostoc mesenteroides |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | 0.079 | NADH | - |
Leuconostoc mesenteroides | |
| 0.18 | 0.27 | NAD+ | - |
Leuconostoc mesenteroides | |
| 0.29 | 21.8 | D-mannitol | - |
Leuconostoc mesenteroides | |
| 16.3 | 79.2 | D-fructose | - |
Leuconostoc mesenteroides | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannitol + NAD+ | Leuconostoc mesenteroides | - |
D-fructose + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leuconostoc mesenteroides | - |
- |
- |
| no activity in Lactobacillus plantarum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannitol + NAD+ | - |
Leuconostoc mesenteroides | D-fructose + NADH + H+ | - |
? | |
| D-mannitol + NAD+ | - |
Leuconostoc mesenteroides | D-fructose + NADH + H+ | - |
r | |
| L-sorbitol + NAD+ | - |
Leuconostoc mesenteroides | L-sorbose + NADH + H+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
D-fructose reduction | Leuconostoc mesenteroides |
| 8.6 | - |
D-mannitol oxidation | Leuconostoc mesenteroides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
not active below, D-mannitol oxidation | Leuconostoc mesenteroides |
| 9 | 9.5 | not active above, D-fructose reduction | Leuconostoc mesenteroides |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Leuconostoc mesenteroides |
| 6 | 8 | - |
Leuconostoc mesenteroides |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Leuconostoc mesenteroides | |
| NADH | - |
Leuconostoc mesenteroides | |
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Release 2023.1 (January 2023)
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