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Literature summary for 1.1.1.67 extracted from

  • Kulbe, K.D.; Schwab, U.; Howaldt, M.; Kimmerle, K.
    Anwendung von Membranverfahren bei der simultanen Herstellung von Mannitol und Gluconsäure aus Saccharose durch konjugierte NAD+-abhängige Dehydrogenasen (1986), GBF Monogr., 9, 189-200.
No PubMed abstract available
Search for more literature for 1.1.1.67

Application

Application Comment Organism
nutrition cofactor regeneration system Saccharomyces cerevisiae

General Stability

General Stability Organism
stabilized with 0.5 mM EDTA, 1% bovine serum albumin Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
D-fructose substrate inhibition, Ki: 1.356 mM Saccharomyces cerevisiae
D-mannitol product inhibition, Ki: 24.6 mM Saccharomyces cerevisiae
L-sorbitol Ki: 24.6 mM Saccharomyces cerevisiae
NAD+ product inhibition, Ki: 0.6 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01 0.079 NADH
-
 Saccharomyces cerevisiae
0.074
-
 polyethylenimine-NH-succinyl-NADH
-
 Saccharomyces cerevisiae
0.1
-
 polyethyleneglycol-NH-succinyl-aminoethyl-NADH
-
 Saccharomyces cerevisiae
0.125
-
 polyethyleneglycol-NH-succinyl-NADH
-
 Saccharomyces cerevisiae
16.3 79.2 D-fructose
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-mannitol + NAD+ Saccharomyces cerevisiae
-
 D-fructose + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 yeast
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + polyethylenimine-NH-succinyl-NADH + H+
-
 Saccharomyces cerevisiae D-mannitol + polyethylenimine-NH-succinyl-NAD+
-
 r
D-mannitol + NAD+
-
 Saccharomyces cerevisiae D-fructose + NADH + H+
-
 ?
D-mannitol + NAD+
-
 Saccharomyces cerevisiae D-fructose + NADH + H+
-
 r
D-mannitol + polyethyleneglycol-NH-succinyl-aminoethyl-NAD+
-
 Saccharomyces cerevisiae D-fructose + polyethyleneglycol-NH-succinyl-aminoethyl-NADH
-
 ?
D-mannitol + polyethyleneglycol-NH-succinyl-NAD+
-
 Saccharomyces cerevisiae D-fructose + polyethyleneglycol-NH-succinyl-NADH
-
 ?
D-mannitol + polyethylenimin-NH-succinyl-NAD+
-
 Saccharomyces cerevisiae D-fructose + polyethylenimin-NH-succinyl-NADH
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
MDH
-
 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.2 D-fructose reduction Saccharomyces cerevisiae
8.9 10 D-mannitol oxidation Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 6.5 highest stability Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Saccharomyces cerevisiae
NADH
-
 Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.6
-
 NAD+
-
 Saccharomyces cerevisiae
24.6
-
 D-mannitol
-
 Saccharomyces cerevisiae
24.6
-
 L-sorbitol
-
 Saccharomyces cerevisiae
1356
-
 D-fructose
-
 Saccharomyces cerevisiae