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Literature summary for 1.1.1.67 extracted from
- Polyol dehydrogenases in the soluble fraction of Acetobacter melanogenum (1968), Agric. Biol. Chem., 32, 161-169.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM, 61% inhibition | Gluconobacter oxydans |  |
| p-hydroxymercuribenzoate | 1 mM, 56% inhibition | Gluconobacter oxydans | |
| Zn2+ | 1 mM, 94% inhibition | Gluconobacter oxydans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannitol + NAD+ | Gluconobacter oxydans | - |
D-fructose + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter oxydans | - |
i.e. Gluconobacter oxydans | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gluconobacter oxydans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
- |
Gluconobacter oxydans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C, 1 month, 50% loss of activity | Gluconobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannitol + NAD+ | - |
Gluconobacter oxydans | D-fructose + NADH + H+ | - |
? | |
| D-mannitol + NAD+ | - |
Gluconobacter oxydans | D-fructose + NADH + H+ | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
heat inactivation, 50% loss of activity after 30 min incubation | Gluconobacter oxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.2 | D-fructose reduction | Gluconobacter oxydans |
| 8.9 | 10 | D-mannitol oxidation | Gluconobacter oxydans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | - |
Gluconobacter oxydans |
| 9 | 9.5 | - |
Gluconobacter oxydans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Gluconobacter oxydans | |
| NADH | - |
Gluconobacter oxydans | |
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Release 2023.1 (January 2023)
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