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Literature summary for 1.1.1.60 extracted from

  • Liu, Y.; Koh, C.; Sun, L.; Ji, L.
    Tartronate semialdehyde reductase defines a novel rate-limiting step in assimilation and bioconversion of glycerol in Ustilago maydis (2011), PLoS ONE, 6, e16438.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Ustilago maydis

Inhibitors

Inhibitors Comment Organism Structure
EDTA severe inhibition Ustilago maydis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00019
-
2-hydroxy-3-oxopropanoate pH 8.5, 40°C Ustilago maydis
0.0003
-
NADP+ pH 8.5, 40°C Ustilago maydis
0.0143
-
NADH pH 8.5, 40°C Ustilago maydis
0.0177
-
D-glycerate pH 8.5, 40°C Ustilago maydis
0.123
-
L-Glycerate pH 8.5, 40°C Ustilago maydis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein Ustilago maydis
41000
-
2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein Ustilago maydis
85000
-
gel filtration Ustilago maydis

Organism

Organism UniProt Comment Textmining
Ustilago maydis A0A0D1DYW4 putative
-

Storage Stability

Storage Stability Organism
4°C, phosphate buffer, pH 7.0, improved solubility Ustilago maydis
4°C, Tris-HCl, pH 8.0, highly unstable, precipitates during storage Ustilago maydis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-3-oxopropanoate + NADH + H+
-
Ustilago maydis D-glycerate + NAD+
-
?
D-glycerate + NADP+ enzyme shows strong specificity and enantioselectivity for D-glycerate Ustilago maydis 2-hydroxy-3-oxopropanoate + NADPH + H+
-
?
L-glycerate + NADP+ enzyme shows strong specificity and enantioselectivity for D-glycerate Ustilago maydis 2-hydroxy-3-oxopropanoate + NADPH + H+
-
?
additional information poor activity towards 6-phosphogluconic acid, beta-hydroxybutyric acid and D-threonine Ustilago maydis ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein Ustilago maydis

Synonyms

Synonyms Comment Organism
TSR1
-
Ustilago maydis
UM02592
-
Ustilago maydis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Ustilago maydis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Ustilago maydis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
rapidly precipitates below Ustilago maydis
7
-
phosphate buffer, pH 7.0, improved solubility Ustilago maydis
8
-
Tris-HCl, highly unstable, precipitates during storage Ustilago maydis

Cofactor

Cofactor Comment Organism Structure
NAD+ about 75% of the activity with NADP+ Ustilago maydis
NADP+ preferred over NAD+ Ustilago maydis

General Information

General Information Comment Organism
physiological function over-expression of the gene results in improved glycerol assimilation. Glycolipid accumulation is reduced by 45.2% in the knockout mutant whereas over-expression of the gene increases it by 40.4% Ustilago maydis