Cloned (Comment) | Organism |
---|---|
native and insertion protein, expressed as His-tag fusion protein in Escherichia coli XL1-blue and BL21 | Comamonas testosteroni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23380 | - |
x * 23380, insertion protein, calculated from the deduced amino acid sequence, confirmed by SDS-PAGE | Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Comamonas testosteroni | catalyzes the oxidoreduction of a variety of steroid substrates, including the steroid antibiotic fusidic acid, catalyzes the interconversion of hydroxy and oxo groups at position 3 of the steroid ring structure, enzyme is capable of catalyzing the carbonyl reduction of non-steroidal xenobiotic carbonyl compounds, contributes to the bioremediation of natural and synthetic toxicants, plays a central role in steroid metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | - |
able to use steroids as sole carbon source | - |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Comamonas testosteroni |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
androstan-3alpha,17beta-diol + NAD+ = 17beta-hydroxyandrostan-3-one + NADH + H+ | catalytic residues are Ser114, Tyr127, and Lys131 | Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | catalyzes the oxidoreduction of a variety of steroid substrates, including the steroid antibiotic fusidic acid, catalyzes the interconversion of hydroxy and oxo groups at position 3 of the steroid ring structure, enzyme is capable of catalyzing the carbonyl reduction of non-steroidal xenobiotic carbonyl compounds, contributes to the bioremediation of natural and synthetic toxicants, plays a central role in steroid metabolism | Comamonas testosteroni | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23380, insertion protein, calculated from the deduced amino acid sequence, confirmed by SDS-PAGE | Comamonas testosteroni |
dimer | crystal structure analysis, in silico data | Comamonas testosteroni |
Synonyms | Comment | Organism |
---|---|---|
3alpha-HSD/CR | - |
Comamonas testosteroni |
3alpha-hydroxysteroid dehydrogenase/carbonyl reductase | belongs to the short chain dehydrogenase/reductase (SDR) protein superfamily | Comamonas testosteroni |