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Literature summary for 1.1.1.53 extracted from

  • Krook, M.; Prozorovski, V.; Atrian, S.; Gonzalez-Duarte, R.; Jörnvall, H.
    Short-chain dehydrogenases. Proteolysis and chemical modification of prokaryotic 3alpha/20beta-hydroxysteroid, insect alcohol and human 15-hydroxyprostaglandin dehydrogenase (1992), Eur. J. Biochem., 209, 233-239.
    View publication on PubMed

General Stability

General Stability Organism
sensitive to proteolysis with Glu-C protease and trypsin, which can be nearly completly hindered by presence of NADH or cortisone Streptomyces exfoliatus

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition with iodoacetate, acetylsialic acid, phenylmethylsulfonyl fluoride, glyoxal, iodoacetamide, dimethylaminoisopropyl ethylene dicarbodiimide, acetylimidazol, diethyl pyrocarbonate Streptomyces exfoliatus
pyridoxal 5'-phosphate slightly Streptomyces exfoliatus
Tetranitromethane other Tyr-selective reagents, Tyr residues are labeled with nitro-group by inhibitor Streptomyces exfoliatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3alpha,20beta-hydroxysteroid androstanes or pregnane derivatives + NAD(P)H Streptomyces exfoliatus enzyme belongs to the group of short-chain dehydrogenases ?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces exfoliatus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3alpha,20beta-hydroxysteroid androstanes or pregnane derivatives + NAD(P)H enzyme belongs to the group of short-chain dehydrogenases Streptomyces exfoliatus ?
-
?
cortisone + NAD(P)H
-
Streptomyces exfoliatus 17alpha,20beta,21-trihydroxy-pregn-4-ene-3,11-dione + NAD(P)+
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
low stability Streptomyces exfoliatus

Cofactor

Cofactor Comment Organism Structure
NADH protects enzyme almost completely against proteolysis with Glu-C protease and trypsin Streptomyces exfoliatus