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Literature summary for 1.1.1.53 extracted from

  • Tyrakowska, B.; Verhaert, R.M.D.; Hilhorst, R.; Veeger, C.
    Enzyme kinetics in reversed micelles. 3. Behaviour of 20beta-hydroxysteroid dehydrogenase (1990), Eur. J. Biochem., 187, 81-88.
    View publication on PubMed
Search for more literature for 1.1.1.53

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011 0.0025 progesterone in reversed micelles, value depends on type of detergent Streptomyces exfoliatus
0.0028 0.005 progesterone in aqueous solution, value increases with increasing ionic strength Streptomyces exfoliatus
0.0035 0.0057 NADH in aqueous solution, value increases with increasing ionic strength Streptomyces exfoliatus
0.004 0.016 NADH in reversed micelles, value depends on type of detergent Streptomyces exfoliatus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Streptomyces exfoliatus 5829
-

Organism

Organism UniProt Comment Textmining
Streptomyces exfoliatus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Streptomyces exfoliatus
-

Storage Stability

Storage Stability Organism
-20°C, 5 mM phosphate buffer, pH 7.6 Streptomyces exfoliatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
progesterone + NAD(P)H
-
 Streptomyces exfoliatus 20beta-hydroxy-pregn-4-ene-3-one + NAD(P)+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 reduction of progesterone in aqueous solution Streptomyces exfoliatus
6.4
-
 reduction of progesterone in reversed micelles Streptomyces exfoliatus

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Streptomyces exfoliatus