Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 10 mM, 50-75% inhibition, assay without MgCl2 | Escherichia coli | |
Cd2+ | 1 mM, 83% inhibition | Escherichia coli | |
Co2+ | 1 mM, 56% inhibition | Escherichia coli | |
Cu2+ | 1 mM, complete inhibition | Escherichia coli | |
Hg2+ | 1 mM, complete inhibition | Escherichia coli | |
Mn2+ | 1 mM, 56% inhibition | Escherichia coli | |
NADPH | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
NADP+ | 25°C, pH 8.0, high-level glucose-6-phosphate dehydrogenase strain DF82 | Escherichia coli | |
0.016 | - |
NADP+ | 25°C, pH 8.0, wild-type strain K-10 | Escherichia coli | |
0.1 | - |
D-glucose 6-phosphate | 25°C, pH 8.0, wild-type strain K-10 | Escherichia coli | |
0.145 | - |
D-glucose 6-phosphate | 25°C, pH 8.0, high-level glucose-6-phosphate dehydrogenase strain DF82 | Escherichia coli | |
1.67 | - |
NAD+ | 25°C, pH 8.0 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 1.4fold activation | Escherichia coli | |
Mg2+ | 1 mM, 1.6fold activation | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | Escherichia coli | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | Escherichia coli K-10 | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | Escherichia coli DF82 | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
high-level glucose-6-phosphate dehydrogenase strain | - |
Escherichia coli DF82 | - |
high-level glucose-6-phosphate dehydrogenase strain | - |
Escherichia coli K-10 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
approximately 100-fold purification | Escherichia coli |
approximately 450fold purification | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
100 | - |
25°C, pH 8.0, high-level glucose-6-phosphate dehydrogenase strain DF82 | Escherichia coli |
104 | - |
25°C, pH 8.0, wild-type strain K-10 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NAD+ | - |
Escherichia coli | 6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NAD+ | - |
Escherichia coli K-10 | 6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NAD+ | - |
Escherichia coli DF82 | 6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | - |
Escherichia coli | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | - |
Escherichia coli K-10 | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | - |
Escherichia coli DF82 | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
additional information | the following substances, 1 mM, are not substrates when substituted for D-glucose 6-phosphate: D-glucose 6-phosphate, D-fructose 1-phosphate, D-gluconate 6-phosphate, D-galactose 6-phosphate, D-ribose 5-phosphate, and D-glucose | Escherichia coli | ? | - |
? | |
additional information | the following substances, 1 mM, are not substrates when substituted for D-glucose 6-phosphate: D-glucose 6-phosphate, D-fructose 1-phosphate, D-gluconate 6-phosphate, D-galactose 6-phosphate, D-ribose 5-phosphate, and D-glucose | Escherichia coli K-10 | ? | - |
? | |
additional information | the following substances, 1 mM, are not substrates when substituted for D-glucose 6-phosphate: D-glucose 6-phosphate, D-fructose 1-phosphate, D-gluconate 6-phosphate, D-galactose 6-phosphate, D-ribose 5-phosphate, and D-glucose | Escherichia coli DF82 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 9.8 | pH 6.2: about 50% of maximal activity, pH 9.8: about 50% of maximal activity | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NAD+ can replace NADP+, with 10 mM NAD+ giving 25% of the rate in the usual assay with NADP+ | Escherichia coli | |
NADP+ | - |
Escherichia coli |