Literature summary for 1.1.1.49 extracted from

Ortiz, C.; Larrieux, N.; Medeiros, A.; Botti, H.; Comini, M.; Buschiazzo, A.
Expression, crystallization and preliminary X-ray crystallographic analysis of glucose-6-phosphate dehydrogenase from the human pathogen Trypanosoma cruzi in complex with substrate (2011), Acta Crystallogr. Sect. F, 67, 1457-1461.

Crystallization (Commentary)

Crystallization (Comment)	Organism
N-terminally truncated enzyme mutant DELTA37N, sitting drop vapour diffusion method, from 20 mMTris-HCl, pH 8.0, and 5 mM D-glucose 6-phosphate, mixing of 0.002 ml reservoir solution, containing 6% PEG 400, 1.6 M ammonium sulfate, 0.1 M HEPES pH 7.5 without D-glucose 6-phosphate and 4% PEG 400, 1.8 M ammonium sulfate, 0.1 M Na HEPES pH 7.5, with D-glucose 6-phosphate, with 0.002 ml of protein solution, containing 20 mg/ml and 33 mg/ml protein, respectively, followed by equilibration against 0.09 ml of reservoir solution, 4 days, X-ray diffraction structure determination and analysis at 2.85-3.35 A resolution, molecular replacement	Trypanosoma cruzi

Crystallization (Comment)

Organism

N-terminally truncated enzyme mutant DELTA37N, sitting drop vapour diffusion method, from 20 mMTris-HCl, pH 8.0, and 5 mM D-glucose 6-phosphate, mixing of 0.002 ml reservoir solution, containing 6% PEG 400, 1.6 M ammonium sulfate, 0.1 M HEPES pH 7.5 without D-glucose 6-phosphate and 4% PEG 400, 1.8 M ammonium sulfate, 0.1 M Na HEPES pH 7.5, with D-glucose 6-phosphate, with 0.002 ml of protein solution, containing 20 mg/ml and 33 mg/ml protein, respectively, followed by equilibration against 0.09 ml of reservoir solution, 4 days, X-ray diffraction structure determination and analysis at 2.85-3.35 A resolution, molecular replacement

Trypanosoma cruzi

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of a N-terminally truncated version of the enzyme glucose-6-phosphate dehydrogenase from Trypanosoma cruzi lacking the first 37 residues, mutant DELTA37N	Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glucose 6-phosphate + NADP+	Trypanosoma cruzi	-	D-glucono-1,5-lactone 6-phosphate + NADPH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma cruzi	Q1WBU6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucose 6-phosphate + NADP+	-	Trypanosoma cruzi	D-glucono-1,5-lactone 6-phosphate + NADPH + H+	-	?

Synonyms

Synonyms	Comment	Organism
G6PDH	-	Trypanosoma cruzi

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Trypanosoma cruzi