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Literature summary for 1.1.1.49 extracted from
- 16-Bromoepiandrosterone, an activator of the mammalian immune system, inhibits glucose 6-phosphate dehydrogenase from Trypanosoma cruzi and is toxic to these parasites grown in culture (2010), Bioorg. Med. Chem., 18, 4762-4768.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli, His-tagged protein | Trypanosoma cruzi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5alpha-androstan-16-alpha-bromo-3beta-ol-17-one | in vitro cell viability assays shows a LD50 of 20 microM for Trypanosoma cruzi epimastigotes | Trypanosoma cruzi |  |
| 5alpha-Androstan-3beta-ol-17-one | uncompetitive inhibitor | Trypanosoma cruzi | |
| 5alpha-androsten-16-alpha-bromo-3beta-ol-17-one | potent inhibitor, in vitro cell viability assays shows a LD50 of 12 microM for Trypanosoma cruzi epimastigotes | Trypanosoma cruzi | |
| D-glucose 6-phosphate | - |
Trypanosoma cruzi | |
| dehydroandrosterone | uncompetitive inhibitor | Trypanosoma cruzi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0225 | - |
NADP+ | pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | |
| 0.206 | - |
D-glucose 6-phosphate | pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | sequential mechanism | Trypanosoma cruzi |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADP+ + H2O | - |
Trypanosoma cruzi | D-gluconate 6-phosphate + NADPH + H+ | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 57.1 | - |
D-glucose 6-phosphate | pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0048 | - |
5alpha-Androstan-3beta-ol-17-one | substrate D-glucose 6-phosphate, pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | |
| 0.0048 | - |
5alpha-Androstan-3beta-ol-17-one | substrate NADP+, pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | |
| 0.0215 | - |
dehydroandrosterone | substrate D-glucose 6-phosphate, pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | |
| 0.0223 | - |
dehydroandrosterone | substrate NADP+, pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | |
| 0.1053 | - |
D-glucose 6-phosphate | pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.000086 | - |
pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | 5alpha-androsten-16-alpha-bromo-3beta-ol-17-one | |
| 0.000216 | - |
pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | 5alpha-androstan-16-alpha-bromo-3beta-ol-17-one | |
| 0.0056 | - |
pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | dehydroandrosterone | |
| 0.025 | - |
pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | 5alpha-Androstan-3beta-ol-17-one | |
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