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Literature summary for 1.1.1.49 extracted from

  • Wang, X.T.; Engel, P.C.
    Clinical mutants of human glucose 6-phosphate dehydrogenase: impairment of NADP(+) binding affects both folding and stability (2009), Biochim. Biophys. Acta, 1792, 804-809.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and clinical mutants expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
G488S clinical mutant G6PDFukaya, mutation in the vicinity of the structural NADP+ site, elevated Kd values of the structural NADP+, is denatured by guanidinium hydrochloride and refolded by rapid dilution in the presence of L-Arg, NADP+ and dithiothreitol at 25°C, displays decreased thermostability and high susceptibility to chymotrypsin digestion as compared to the wild-type Homo sapiens
G488V clinical mutant G6PDCampinas, mutation in the vicinity of the structural NADP+ site, elevated Kd values of the structural NADP+, is denatured by guanidinium hydrochloride and refolded by rapid dilution in the presence of L-Arg, NADP+ and dithiothreitol at 25°C, displays decreased thermostability and high susceptibility to chymotrypsin digestion as compared to the wild-type Homo sapiens
R393G clinical mutant G6PDWisconsin, mutation in the vicinity of the structural NADP+ site, elevated Kd values of the structural NADP+, is denatured by guanidinium hydrochloride and refolded by rapid dilution in the presence of L-Arg, NADP+ and dithiothreitol at 25°C, displays thermostability as the wild-type and low susceptibility to chymotrypsin digestion Homo sapiens
R393H clinical mutant G6PDNashville, mutation in the vicinity of the structural NADP+ site, elevated Kd values of the structural NADP+, is denatured by guanidinium hydrochloride and refolded by rapid dilution in the presence of L-Arg, NADP+ and dithiothreitol at 25°C, displays decreased thermostability and high susceptibility to chymotrypsin digestion as compared to the wild-type Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
chymotrypsin
-
Homo sapiens
guanidinium hydrochloride denatures Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0107
-
NADP+ clinical mutant G488V Homo sapiens
0.0118
-
NADP+ clinical mutant G488S Homo sapiens
0.0491
-
D-glucose 6-phosphate clinical mutant G488S Homo sapiens
0.0538
-
D-glucose 6-phosphate clinical mutant G488V Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and clinical mutants purified to homogeneity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + NADP+
-
Homo sapiens D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
G6PD
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
212
-
D-glucose 6-phosphate clinical mutant G488V Homo sapiens
254
-
D-glucose 6-phosphate clinical mutant G488S Homo sapiens