Cloned (Comment) | Organism |
---|---|
the two variants G6PD(Plymouth) (G163D) and G6PD(Mahidol) (G163S) are constructed by site-directed mutagenesis and expressed in G6PD-deficient Escherichia coli DF 213 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G163D | mutant is markedly less stable than wild-type G6PD in both thermostability and urea-induced inactivation tests. According to unfolding and refolding experiments, the mutant is impaired in its folding properties. KM-values and turnover numbers are similar to wild-type values | Homo sapiens |
G163S | mutant markedly less stable than wild-type G6PD in both thermostability and urea-induced inactivation tests. According to unfolding and refolding experiments, the mutant is impaired in its folding properties. KM-values and turnover numbers are similar to wild-type values | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.67 | - |
NADP+ | 25°C, pH 8.0, wild-type enzyme | Homo sapiens | |
4.8 | - |
NADP+ | 25°C, pH 8.0, mutant enzyme G163D | Homo sapiens | |
6.46 | - |
NADP+ | 25°C, pH 8.0, mutant enzyme G163S | Homo sapiens | |
44.4 | - |
D-glucose 6-phosphate | 25°C, pH 8.0, mutant enzyme G163D | Homo sapiens | |
45.8 | - |
D-glucose 6-phosphate | 25°C, pH 8.0, wild-type enzyme | Homo sapiens | |
50.7 | - |
D-glucose 6-phosphate | 25°C, pH 8.0, mutant enzyme G163S | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P11413 | - |
- |
Purification (Comment) | Organism |
---|---|
two varients G6PD(Plymouth) (G163D) and G6PD(Mahidol) (G163S) | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | - |
Homo sapiens | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
pH 7.2, 24 h: wild-type enzyme loses 42% of its initial activity, mutant enzyme G163S loses 73% of its initial activity, mutant enzyme G163D loses 94% of its initial activity. Stability of all three enzymes is enhanced by addition of NADP+ | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
207 | - |
NADP+ | 25°C, pH 8.0, mutant enzyme G163S | Homo sapiens | |
207 | - |
D-glucose 6-phosphate | 25°C, pH 8.0, mutant enzyme G163S | Homo sapiens | |
249 | - |
NADP+ | 25°C, pH 8.0, mutant enzyme G163D | Homo sapiens | |
249 | - |
D-glucose 6-phosphate | 25°C, pH 8.0, mutant enzyme G163D | Homo sapiens | |
251 | - |
NADP+ | 25°C, pH 8.0, wild-type enzyme | Homo sapiens | |
251 | - |
D-glucose 6-phosphate | 25°C, pH 8.0, wild-type enzyme | Homo sapiens |