Literature summary for 1.1.1.49 extracted from

Huang, Y.; Choi, M.Y.; Au, S.W.; Au, D.M.; Lam, V.M.; Engel, P.C.
Purification and detailed study of two clinically different human glucose 6-phosphate dehydrogenase variants, G6PD(Plymouth) and G6PD(Mahidol): Evidence for defective protein folding as the basis of disease (2008), Mol. Genet. Metab., 93, 44-53.

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Cloned(Commentary)

Cloned (Comment)	Organism
the two variants G6PD(Plymouth) (G163D) and G6PD(Mahidol) (G163S) are constructed by site-directed mutagenesis and expressed in G6PD-deficient Escherichia coli DF 213 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
G163D	mutant is markedly less stable than wild-type G6PD in both thermostability and urea-induced inactivation tests. According to unfolding and refolding experiments, the mutant is impaired in its folding properties. KM-values and turnover numbers are similar to wild-type values	Homo sapiens
G163S	mutant markedly less stable than wild-type G6PD in both thermostability and urea-induced inactivation tests. According to unfolding and refolding experiments, the mutant is impaired in its folding properties. KM-values and turnover numbers are similar to wild-type values	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.67	-	NADP+	25°C, pH 8.0, wild-type enzyme	Homo sapiens
4.8	-	NADP+	25°C, pH 8.0, mutant enzyme G163D	Homo sapiens
6.46	-	NADP+	25°C, pH 8.0, mutant enzyme G163S	Homo sapiens
44.4	-	D-glucose 6-phosphate	25°C, pH 8.0, mutant enzyme G163D	Homo sapiens
45.8	-	D-glucose 6-phosphate	25°C, pH 8.0, wild-type enzyme	Homo sapiens
50.7	-	D-glucose 6-phosphate	25°C, pH 8.0, mutant enzyme G163S	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P11413	-	-

Purification (Commentary)

Purification (Comment)	Organism
two varients G6PD(Plymouth) (G163D) and G6PD(Mahidol) (G163S)	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucose 6-phosphate + NADP+	-	Homo sapiens	D-glucono-1,5-lactone 6-phosphate + NADPH + H+	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
37	-	pH 7.2, 24 h: wild-type enzyme loses 42% of its initial activity, mutant enzyme G163S loses 73% of its initial activity, mutant enzyme G163D loses 94% of its initial activity. Stability of all three enzymes is enhanced by addition of NADP+	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
207	-	NADP+	25°C, pH 8.0, mutant enzyme G163S	Homo sapiens
207	-	D-glucose 6-phosphate	25°C, pH 8.0, mutant enzyme G163S	Homo sapiens
249	-	NADP+	25°C, pH 8.0, mutant enzyme G163D	Homo sapiens
249	-	D-glucose 6-phosphate	25°C, pH 8.0, mutant enzyme G163D	Homo sapiens
251	-	NADP+	25°C, pH 8.0, wild-type enzyme	Homo sapiens
251	-	D-glucose 6-phosphate	25°C, pH 8.0, wild-type enzyme	Homo sapiens

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Release 2023.1 (January 2023)