Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Aspergillus aculeatus | |
ATP | - |
Aspergillus aculeatus | |
Co2+ | competitive, 60% inhibition at 0.01 mM | Aspergillus aculeatus | |
fructose 6-phosphate | - |
Aspergillus aculeatus | |
additional information | product and dead-end inhibition studies, overview, no inhibition by AMP | Aspergillus aculeatus | |
NADP+ | substrate inhibition | Aspergillus aculeatus | |
NADPH | product inhibition | Aspergillus aculeatus | |
phosphoenolpyruvate | - |
Aspergillus aculeatus | |
Zn2+ | competitive, 40% inhibition at 0.01 mM | Aspergillus aculeatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, kinetic mechanism analysis, ternary-complex mechanism, overview | Aspergillus aculeatus | |
0.006 | - |
NADP+ | pH 7.5 | Aspergillus aculeatus | |
0.075 | - |
D-glucose 6-phosphate | pH 7.5 | Aspergillus aculeatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Aspergillus aculeatus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
2 * 52000, SDS-PAGE | Aspergillus aculeatus |
105000 | - |
gel filtration | Aspergillus aculeatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | Aspergillus aculeatus | inhibition by divalent metal ions, at low NADPH /NADP+ ratio, is another means of controlling the pentose phosphate pathway | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus aculeatus | - |
filamentous fungus isolated from infected tongue of a human patient | - |
Purification (Comment) | Organism |
---|---|
native enzyme 230fold to homogeneity by ammonium sulfate fractionation, affinity chromatography, and gel filtration | Aspergillus aculeatus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | ternary-complex mechanism | Aspergillus aculeatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus aculeatus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
220 | - |
purified enzyme | Aspergillus aculeatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | inhibition by divalent metal ions, at low NADPH /NADP+ ratio, is another means of controlling the pentose phosphate pathway | Aspergillus aculeatus | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | the substrate specificity is extremely strict | Aspergillus aculeatus | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
additional information | no activity with glucose and glucosamine 6-phosphate | Aspergillus aculeatus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52000, SDS-PAGE | Aspergillus aculeatus |
Synonyms | Comment | Organism |
---|---|---|
G6PD | - |
Aspergillus aculeatus |
glucose 6-phosphate dehydrogenase | - |
Aspergillus aculeatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
purified enzyme, rapid and irreversible inactivation, half life: 3.2 min, in presence of substrate D-glucose 6-phosphate 7.5 min | Aspergillus aculeatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
83 | - |
D-glucose 6-phosphate | pH 7.5 | Aspergillus aculeatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Aspergillus aculeatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | specific for, no activity with NAD+ | Aspergillus aculeatus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0047 | - |
Co2+ | pH 7.5 | Aspergillus aculeatus | |
0.0066 | - |
Zn2+ | pH 7.5 | Aspergillus aculeatus | |
0.02 | - |
NADPH | pH 7.5, versus NADP+ | Aspergillus aculeatus | |
0.023 | - |
NADP+ | pH 7.5 | Aspergillus aculeatus | |
0.041 | 0.043 | NADPH | pH 7.5, versus D-glucose 6-phosphate | Aspergillus aculeatus |