Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R393E | site-directed mutagenesis, the mutation affects a residue in the dimer interface close to the structural NADP+ site, the mutant activity is slightly reduced compared to the activity of the wild-type enzyme | Homo sapiens |
R393G | naturally occuring mutation corresponding to the clinical variants G6PD Wisconsin, the mutation affects a residue in the dimer interface close to the structural NADP+ site, the mutant activity is similar to the activity of the wild-type enzyme | Homo sapiens |
R393H | naturally occuring mutation corresponding to the clinical variants G6PD Nashville, the mutation affects a residue in the dimer interface close to the structural NADP+ site, the mutant activity is reduced compared to the activity of the wild-type enzyme | Homo sapiens |
R393I | site-directed mutagenesis, the mutation affects a residue in the dimer interface close to the structural NADP+ site, the mutant activity is similar to the activity of the wild-type enzyme | Homo sapiens |
R393L | site-directed mutagenesis, the mutation affects a residue in the dimer interface close to the structural NADP+ site, the mutant activity is similar to the activity of the wild-type enzyme | Homo sapiens |
R393V | site-directed mutagenesis, the mutation affects a residue in the dimer interface close to the structural NADP+ site, the mutant activity is reduced compared to the activity of the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics of wild-type and mutant enzymes | Homo sapiens | |
0.0071 | - |
NADP+ | pH 8.0, 25°C, recombinant wild-type enzyme | Homo sapiens | |
0.0093 | - |
NADP+ | pH 8.0, 25°C, recombinant mutant R393G | Homo sapiens | |
0.0165 | - |
NADP+ | pH 8.0, 25°C, recombinant mutant R393H | Homo sapiens | |
0.052 | - |
D-glucose 6-phosphate | pH 8.0, 25°C, recombinant wild-type enzyme | Homo sapiens | |
0.067 | - |
D-glucose 6-phosphate | pH 8.0, 25°C, recombinant mutant R393G | Homo sapiens | |
0.19 | - |
D-glucose 6-phosphate | pH 8.0, 25°C, recombinant mutant R393H | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | Homo sapiens | - |
D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by 2',5'-ADP affinity chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
130 | - |
purified recombinant mutant R393H | Homo sapiens |
135 | - |
purified recombinant mutant R393E | Homo sapiens |
166 | - |
purified recombinant mutant R393V | Homo sapiens |
176 | - |
purified recombinant mutant R393L | Homo sapiens |
178 | - |
purified recombinant mutant R393G | Homo sapiens |
180 | - |
purified recombinant wild-type enzyme | Homo sapiens |
181 | - |
purified recombinant mutant R393I | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | - |
Homo sapiens | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G6PD | - |
Homo sapiens |
glucose 6-phosphate dehydrogenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
192 | - |
D-glucose 6-phosphate | pH 8.0, 25°C, recombinant mutant R393H | Homo sapiens | |
232 | - |
D-glucose 6-phosphate | pH 8.0, 25°C, recombinant mutant R393G | Homo sapiens | |
275 | - |
D-glucose 6-phosphate | pH 8.0, 25°C, recombinant wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens |