General Stability | Organism |
---|---|
isoform A(-) is less stable during purification than A(+) due to V68M mutation that makes it suceptible to oxidation of sulfhydryl group | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + H2O + NADP+ | Homo sapiens | isoform A(-) shows sigmoidal enzyme-substrate interaction, isoform A(+) shows hyperbolic enzyme-substrate interaction | D-gluconate 6-phosphate + NADPH + H+ | D-glucose-delta-lactone 6-phosphate | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
2 isoforms: A(+) and A(-), where A(-) is a mutant derivative of A(+) with V68M exchange | - |
Purification (Comment) | Organism |
---|---|
both isoforms A(-) and A(+) | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
isoform A(-) shows about ten times lower specific activity than isoform A(+) | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + H2O + NADP+ | isoform A(-) shows sigmoidal enzyme-substrate interaction, isoform A(+) shows hyperbolic enzyme-substrate interaction | Homo sapiens | D-gluconate 6-phosphate + NADPH + H+ | D-glucose-delta-lactone 6-phosphate | r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
isoform A(+) | Homo sapiens |
9 | - |
isoform A(-) | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | isoform A(-) has a higher affinity for NADP+ | Homo sapiens |