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Literature summary for 1.1.1.431 extracted from

  • Nidetzky, B.; Brueggler, K.; Kratzer, R.; Mayr, P.
    Multiple forms of xylose reductase in Candida intermedia comparison of their functional properties using quantitative structure-activity relationships, steady-state kinetic analysis, and pH studies (2003), J. Agric. Food Chem., 51, 7930-7935 .
    View publication on PubMed
Search for more literature for 1.1.1.431

Application

Application Comment Organism
synthesis dual specific xylose reductase (dsXR) has an about 4fold higher specificity for NADH than NADPH. This fact could make this enzyme an interesting candidate to be used in metabolic engineering of the yeast xylose metabolism, likely in Saccharomyces cerevisiae. Increased levels of dsXR activity could contribute to an improvement of ethanol production from D-xylose by reducing the cofactor imbalance of the initial catabolic pathway [Candida] intermedia

Inhibitors

Inhibitors Comment Organism Structure
NADP+ competitive inhibitor against NADPH; for monospecific xylose reductase (msXR) and dual specific xylose reductase (dsXR) NADP+ behaves as a competitive inhibitor against NADPH. Competitive inhibition of is observed both at unsaturating and saturating concentrations of xylose [Candida] intermedia
NADPH competitive inhibitor against NADP+; for monospecific xylose reductase (msXR) and dual specific xylose reductase (dsXR) NADPH behaves as a competitive inhibitor against NADP+. Competitive inhibition of is observed both at unsaturating and saturating concentrations of xylitol [Candida] intermedia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 D-glucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
0.01
-
 D-fucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
0.016
-
 NADP+ pH 7, 25°C [Candida] intermedia
0.016
-
 D-xylose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
0.02
-
 L-arabinose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
0.028
-
 NADPH pH 7, 25°C [Candida] intermedia
0.033
-
 D-erythrose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
0.038
-
 2-deoxy-D-ribose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
0.058
-
 2-deoxy-D-glucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
0.061
-
 D-galactose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
0.091
-
 D-ribose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
0.117
-
 L-Lyxose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
0.221
-
 2-deoxy-D-galactose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1.14
-
 DL-glyceraldehyde pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
2 3 Butanal pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
3.9
-
 pentanal pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
77
-
 D-xylose pH 7, 25°C [Candida] intermedia
78
-
 propionaldehyde pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
480
-
 xylitol pH 7, 25°C [Candida] intermedia

Organism

Organism UniProt Comment Textmining
[Candida] intermedia
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-galactose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
2-deoxy-D-glucose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
2-deoxy-D-ribose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
butanal + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
D-erythrose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
D-fucose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
D-galactose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
D-glucose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
D-lyxose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
D-ribose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
D-xylose + NADH + H+ reaction is catalyzed by dual specific xylose reductase (dsXR), reaction is not catalyzed by NADPH-dependent monospecific xylose reductase (msXR) [Candida] intermedia xylitol + NAD+
-
 ?
D-xylose + NADPH + H+
-
 [Candida] intermedia xylitol + NADP+
-
 r
D-xylose + NADPH + H+ reaction is catalyzed by NADPH-dependent monospecific xylose reductase (msXR), and by dual specific xylose reductase (dsXR) [Candida] intermedia xylitol + NADP+
-
 ?
DL-glyceraldehyde + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia glycerol + NADP+
-
 ?
L-arabinose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia L-arabinitol + NADP+
-
 ?
L-lyxose + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
additional information Candida intermedia produces two isoforms of xylose reductase: one is NADPH-dependent (monospecific xylose reductase, msXR), and another prefers NADH about 4fold over NADPH (dual specific xylose reductase, dsXR) [Candida] intermedia ?
-
-
pentanal + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
propionaldehyde + NADPH + H+ NADPH-dependent monospecific xylose reductase [Candida] intermedia ?
-
 ?
xylitol + NADP+
-
 [Candida] intermedia D-xylose + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
monospecific xylose reductase
-
 [Candida] intermedia
msXR
-
 [Candida] intermedia
msXR Candida intermedia produces two isoforms of xylose reductase: one is NADPH-dependent (monospecific xylose reductase, msXR), and another prefers NADH about 4fold over NADPH (dual specific xylose reductase, dsXR, cf. 1.1.1.430) [Candida] intermedia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at [Candida] intermedia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
 xylitol pH 7, 25°C [Candida] intermedia
1.4
-
 2-deoxy-D-ribose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
2 8 D-xylose pH 7, 25°C [Candida] intermedia
6.8
-
 2-deoxy-D-glucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
6.9
-
 propionaldehyde pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
8.2
-
 D-glucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
8.4
-
 2-deoxy-D-galactose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
12.2
-
 D-ribose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
15.2
-
 D-galactose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
18.4
-
 L-Lyxose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
20.7
-
 pentanal pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
20.7
-
 D-fucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
21.2
-
 Butanal pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
23.5
-
 D-xylose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
24.3
-
 DL-glyceraldehyde pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
24.3
-
 D-erythrose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
24.5
-
 L-arabinose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at [Candida] intermedia

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 [Candida] intermedia
NADPH
-
 [Candida] intermedia
NADPH dual specific xylose reductase (dsXR) has an about 4fold higher specificity for NADH than NADPH. NADPH-dependent monospecific xylose reductase (msXR) shows non activity with NADH [Candida] intermedia

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.02
-
 NADPH pH 7, 25°C [Candida] intermedia
0.02
-
 NADPH pH 7.0, 25°C, monospecific xylose reductase (msXR) [Candida] intermedia
0.025
-
 NADP+ pH 7, 25°C [Candida] intermedia
0.025
-
 NADP+ pH 7.0, 25°C, monospecific xylose reductase (msXR) [Candida] intermedia

General Information

General Information Comment Organism
metabolism ordered mechanism in which coenzyme binds first and substrate second [Candida] intermedia

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0013
-
 xylitol pH 7, 25°C [Candida] intermedia
0.09
-
 propionaldehyde pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
0.36
-
 D-xylose pH 7, 25°C [Candida] intermedia
0.93
-
 Butanal pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
5.31
-
 pentanal pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
21.3
-
 DL-glyceraldehyde pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
36.8
-
 2-deoxy-D-ribose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
38
-
 2-deoxy-D-galactose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
117
-
 2-deoxy-D-glucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
134
-
 D-ribose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
157
-
 L-Lyxose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
249
-
 D-galactose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
736
-
 D-erythrose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1230
-
 L-arabinose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1370
-
 D-glucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
1470
-
 D-xylose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia
2070
-
 D-fucose pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH [Candida] intermedia