Cloned (Comment) | Organism |
---|---|
gene icd-D or icd-I, genetic organization of ICD isozymes, recombinant overexpression of His-tagged isozyme IDH-I in IDH-deficient Escherichia coli strain DEK2004 | Colwellia psychrerythraea |
gene icd-M or icd-II, genetic organization of ICD isozymes, recombinant overexpression of His-tagged isozyme IDH-IIa in IDH-deficient Escherichia coli strain DEK2004 | Colwellia psychrerythraea |
gene icd-M or icd-II, genetic organization of ICD isozymes, recombinant overexpression of His-tagged isozyme IDH-IIb in IDH-deficient Escherichia coli strain DEK2004 | Colwellia psychrerythraea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Colwellia psychrerythraea | |
Mn2+ | activates | Colwellia psychrerythraea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Colwellia psychrerythraea | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
isocitrate + NADP+ | Colwellia psychrerythraea DSM 8813 | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Colwellia psychrerythraea | Q1XIQ8 | - |
- |
Colwellia psychrerythraea | Q1XIQ9 | - |
- |
Colwellia psychrerythraea DSM 8813 | Q1XIQ8 | - |
- |
Colwellia psychrerythraea DSM 8813 | Q1XIQ9 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | no cell growth at 15-37°C | Colwellia psychrerythraea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Colwellia psychrerythraea | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
isocitrate + NADP+ | - |
Colwellia psychrerythraea DSM 8813 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Colwellia psychrerythraea |
monomer | - |
Colwellia psychrerythraea |
Synonyms | Comment | Organism |
---|---|---|
IDH | - |
Colwellia psychrerythraea |
IDH-I | - |
Colwellia psychrerythraea |
IDH-IIa | - |
Colwellia psychrerythraea |
IDH-IIb | - |
Colwellia psychrerythraea |
NADP+-dependent isocitrate dehydrogenase | - |
Colwellia psychrerythraea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Colwellia psychrerythraea |
30 | - |
assay at | Colwellia psychrerythraea |
50 | - |
optimum of the IDH overall activity in Colwellia psychrerythraea crude cell extract | Colwellia psychrerythraea |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
isozyme IDH-IIa is a cold-adapted enzyme | Colwellia psychrerythraea |
additional information | - |
isozyme IDH-IIa is a mesophilic enzyme | Colwellia psychrerythraea |
additional information | - |
isozyme IDH-IIb is a cold-adapted enzyme | Colwellia psychrerythraea |
10 | 60 | activity range of the IDH overall activity in Colwellia psychrerythraea crude cell extract, inactivation above | Colwellia psychrerythraea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 15 | stable at, purified recombinant His-tagged isozyme IDH-IIa | Colwellia psychrerythraea |
10 | 15 | stable at, purified recombinant His-tagged isozyme IDH-IIb | Colwellia psychrerythraea |
40 | - |
inactivation, purified recombinant His-tagged isozyme IDH-IIa | Colwellia psychrerythraea |
40 | - |
inactivation, purified recombinant His-tagged isozyme IDH-IIb | Colwellia psychrerythraea |
40 | - |
stable up to, purified recombinant His-tagged isozyme IDH-I | Colwellia psychrerythraea |
50 | - |
inactivation, purified recombinant His-tagged isozyme IDH-I | Colwellia psychrerythraea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Colwellia psychrerythraea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Colwellia psychrerythraea | |
NADPH | - |
Colwellia psychrerythraea |
General Information | Comment | Organism |
---|---|---|
evolution | three NADP+-dependent isocitrate dehydrogenase (IDH) isozymes of a psychrophilic bacterium, Colwellia psychrerythraea strain 34H, are analyzed: two monomeric (IDH-IIa and IDH-IIb) and one dimeric (IDH-I) IDHs | Colwellia psychrerythraea |