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Literature summary for 1.1.1.42 extracted from

  • Suzuki, K.; Takada, Y.
    Characterization of NADP(+)-dependent isocitrate dehydrogenase isozymes from a psychrophilic bacterium, Colwellia psychrerythraea strain 34H (2016), Biosci. Biotechnol. Biochem., 80, 1492-1498.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene icd-D or icd-I, genetic organization of ICD isozymes, recombinant overexpression of His-tagged isozyme IDH-I in IDH-deficient Escherichia coli strain DEK2004 Colwellia psychrerythraea
gene icd-M or icd-II, genetic organization of ICD isozymes, recombinant overexpression of His-tagged isozyme IDH-IIa in IDH-deficient Escherichia coli strain DEK2004 Colwellia psychrerythraea
gene icd-M or icd-II, genetic organization of ICD isozymes, recombinant overexpression of His-tagged isozyme IDH-IIb in IDH-deficient Escherichia coli strain DEK2004 Colwellia psychrerythraea

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Colwellia psychrerythraea
Mn2+ activates Colwellia psychrerythraea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Colwellia psychrerythraea
-
2-oxoglutarate + CO2 + NADPH + H+
-
r
isocitrate + NADP+ Colwellia psychrerythraea DSM 8813
-
2-oxoglutarate + CO2 + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Colwellia psychrerythraea Q1XIQ8
-
-
Colwellia psychrerythraea Q1XIQ9
-
-
Colwellia psychrerythraea DSM 8813 Q1XIQ8
-
-
Colwellia psychrerythraea DSM 8813 Q1XIQ9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information no cell growth at 15-37°C Colwellia psychrerythraea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
Colwellia psychrerythraea 2-oxoglutarate + CO2 + NADPH + H+
-
r
isocitrate + NADP+
-
Colwellia psychrerythraea DSM 8813 2-oxoglutarate + CO2 + NADPH + H+
-
r

Subunits

Subunits Comment Organism
homodimer
-
Colwellia psychrerythraea
monomer
-
Colwellia psychrerythraea

Synonyms

Synonyms Comment Organism
IDH
-
Colwellia psychrerythraea
IDH-I
-
Colwellia psychrerythraea
IDH-IIa
-
Colwellia psychrerythraea
IDH-IIb
-
Colwellia psychrerythraea
NADP+-dependent isocitrate dehydrogenase
-
Colwellia psychrerythraea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Colwellia psychrerythraea
30
-
assay at Colwellia psychrerythraea
50
-
optimum of the IDH overall activity in Colwellia psychrerythraea crude cell extract Colwellia psychrerythraea

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
isozyme IDH-IIa is a cold-adapted enzyme Colwellia psychrerythraea
additional information
-
isozyme IDH-IIa is a mesophilic enzyme Colwellia psychrerythraea
additional information
-
isozyme IDH-IIb is a cold-adapted enzyme Colwellia psychrerythraea
10 60 activity range of the IDH overall activity in Colwellia psychrerythraea crude cell extract, inactivation above Colwellia psychrerythraea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 15 stable at, purified recombinant His-tagged isozyme IDH-IIa Colwellia psychrerythraea
10 15 stable at, purified recombinant His-tagged isozyme IDH-IIb Colwellia psychrerythraea
40
-
inactivation, purified recombinant His-tagged isozyme IDH-IIa Colwellia psychrerythraea
40
-
inactivation, purified recombinant His-tagged isozyme IDH-IIb Colwellia psychrerythraea
40
-
stable up to, purified recombinant His-tagged isozyme IDH-I Colwellia psychrerythraea
50
-
inactivation, purified recombinant His-tagged isozyme IDH-I Colwellia psychrerythraea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Colwellia psychrerythraea

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Colwellia psychrerythraea
NADPH
-
Colwellia psychrerythraea

General Information

General Information Comment Organism
evolution three NADP+-dependent isocitrate dehydrogenase (IDH) isozymes of a psychrophilic bacterium, Colwellia psychrerythraea strain 34H, are analyzed: two monomeric (IDH-IIa and IDH-IIb) and one dimeric (IDH-I) IDHs Colwellia psychrerythraea