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Literature summary for 1.1.1.42 extracted from

  • Kobayashi, K.; Hattori, T.; Hayashi, R.; Kirimura, K.
    Overexpression of the NADP+-specific isocitrate dehydrogenase gene (icdA) in citric acid-producing Aspergillus niger WU-2223L (2014), Biosci. Biotechnol. Biochem., 78, 1246-1253.
    View publication on PubMed

Application

Application Comment Organism
biotechnology the icdA gene is a potentially valuable tool for modulating citric acid production by metabolic engineering Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
icdA, recombinant overexpression in Aspergillus niger strain WU-2223L Aspergillus niger

Protein Variants

Protein Variants Comment Organism
additional information construction of an NADP+-ICDH gene (icdA)-overexpressing strain (OPI-1) using Aspergillus niger strain WU-2223L. The amount of citric acid produced by Aspergillus niger can be altered with the NADP+-ICDH activity. Time-dependent transcriptional changes in the genes encoding NADP+-ICDH, NAD+-ICDH, and ICL are analyzed by RT-PCR. The glyoxylate cycle is poorly activated or slightly repressed in the icdA-overexpressing Aspergillus niger clone Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Aspergillus niger
-
2-oxoglutarate + CO2 + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Aspergillus niger
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
Aspergillus niger 2-oxoglutarate + CO2 + NADPH + H+
-
r

Synonyms

Synonyms Comment Organism
icdA
-
Aspergillus niger
NADP+-ICDH
-
Aspergillus niger
NADP+-specific isocitrate dehydrogenase
-
Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Aspergillus niger
NADPH
-
Aspergillus niger

General Information

General Information Comment Organism
metabolism in the tricarboxylic acid cycle, NADP+-specific isocitrate dehydrogenase catalyzes oxidative decarboxylation of isocitric acid to form 2-oxoglutaric acid with NADP+ as a cofactor. NADP+-ICDH may work independently of NAD+-ICDH, EC 1.1.1.41, as one of the TCA cycle regulatory enzymes, especially in relation to citric acid production by Aspergillus niger Aspergillus niger
physiological function ICDH is a key enzyme that regulates the TCA cycle Aspergillus niger