KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0444 | - |
NADP+ | pH 8.0, 70°C | Aeropyrum pernix | |
0.0552 | - |
NADP+ | pH 8.0, 70°C, Km-value for NAD+ is about 140fold higher than the KM-value for NADP+ | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45400 | - |
2 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence | Thermotoga maritima |
45400 | - |
4 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence | Thermotoga maritima |
47900 | - |
2 * 47900, calculated from sequence | Aeropyrum pernix |
68000 | - |
dimer, analytical ultracentrifugation | Thermotoga maritima |
70000 | - |
analytical ultracentrifugation | Aeropyrum pernix |
174000 | - |
tetramer, analytical ultracentrifugation | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Thermotoga maritima | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | Aeropyrum pernix | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | Aeropyrum pernix DSM 11879 | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | Thermotoga maritima DSM 3109 | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9YE81 | - |
- |
Aeropyrum pernix DSM 11879 | Q9YE81 | - |
- |
Thermotoga maritima | Q9X0N2 | - |
- |
Thermotoga maritima DSM 3109 | Q9X0N2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
- |
Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Thermotoga maritima | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | - |
Aeropyrum pernix | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | low activity with NAD+. Km-value for NAD+ is about 140fold higher than the KM-value for NADP+ | Thermotoga maritima | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | - |
Aeropyrum pernix DSM 11879 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | - |
Thermotoga maritima DSM 3109 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | low activity with NAD+. Km-value for NAD+ is about 140fold higher than the KM-value for NADP+ | Thermotoga maritima DSM 3109 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence | Thermotoga maritima |
homodimer | 2 * 47900, calculated from sequence | Aeropyrum pernix |
tetramer | 4 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
NADP-IDH | - |
Thermotoga maritima |
NADP-IDH | - |
Aeropyrum pernix |
TM1148 | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermotoga maritima |
95 | - |
above | Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal denaturation is an irreversible process | Thermotoga maritima |
additional information | - |
thermal denaturation is an irreversible process | Aeropyrum pernix |
98 | - |
the Tm-value is 98.3°C | Thermotoga maritima |
110 | - |
the Tm-value is 109.9°C | Aeropyrum pernix |