Any feedback?
Literature summary for 1.1.1.42 extracted from
- Functional characterization of NADP-dependent isocitrate dehydrogenase isozymes from Trypanosoma cruzi (2011), Mol. Biochem. Parasitol., 177, 61-64.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Tc00.1047053506925.319 or IDH2, recombinant expression of the N-terminally His6-tagged enzyme in Escherichia coli | Trypanosoma cruzi |
| gene Tc00.1047053511575.60 or IDH1, recombinant expression of the N-terminally His6-tagged enzyme in Escherichia coli, co-expression with bacterial GroEL/GroES chaperones is required for IDH1 in order to achieve a soluble enzyme | Trypanosoma cruzi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, overview | Trypanosoma cruzi | |
| 0.021 | - |
DL-isocitrate | pH and temperature not specified in the publication | Trypanosoma cruzi |  |
| 0.045 | - |
NADP+ | pH and temperature not specified in the publication | Trypanosoma cruzi | |
| 0.054 | - |
DL-isocitrate | pH and temperature not specified in the publication | Trypanosoma cruzi | |
| 0.056 | - |
NADP+ | pH and temperature not specified in the publication | Trypanosoma cruzi | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | IDH2 contains a C-terminal PTS1 signal, but this enzyme is mostly localized in the cytosol, expression levels of the cytosolic isoform are significantly increased in amastigotes, metacyclic trypomastigotes and cell-derived trypomastigotes compared to epimastigotes | Trypanosoma cruzi | 5829 | - |
| glycosome | IDH2 contains a C-terminal PTS1 signal, but this enzyme is mostly localized in the cytosol of epimastigotes | Trypanosoma cruzi | 20015 | - |
| mitochondrion | mitochondrial targeting peptide signal is predicted for IDH1 | Trypanosoma cruzi | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Trypanosoma cruzi | |
| Mn2+ | activates, preferred divalent cation | Trypanosoma cruzi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, recombinant N-terminally His6-tagged enzyme, SDS-PAGE | Trypanosoma cruzi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DL-isocitrate + NADP+ | Trypanosoma cruzi | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | Q4DG65 | gene Tc00.1047053511575.60 or IDH1; gene idh, isozyme IDH1 | - |
| Trypanosoma cruzi | Q4E4L7 | gene Tc00.1047053506925.319 or IDH2; gene idh, two isozymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Trypanosoma cruzi |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| amastigote | - |
Trypanosoma cruzi | - |
| epimastigote | - |
Trypanosoma cruzi | - |
| additional information | expression levels of the cytosolic isoform are significantly increased in amastigotes, metacyclic trypomastigotes and cell-derived trypomastigotes compared to epimastigotes | Trypanosoma cruzi | - |
| trypomastigote | - |
Trypanosoma cruzi | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 67 | - |
purified recombinant N-terminally His6-tagged enzyme, pH and temperature not specified in the publication | Trypanosoma cruzi |
| 219 | - |
purified recombinant N-terminally His6-tagged enzyme, pH and temperature not specified in the publication | Trypanosoma cruzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DL-isocitrate + NADP+ | - |
Trypanosoma cruzi | 2-oxoglutarate + CO2 + NADPH + H+ | - |
ir | |
| DL-isocitrate + NADP+ | carboxylation of 2-oxoglutarate to produce isocitrate | Trypanosoma cruzi | 2-oxoglutarate + CO2 + NADPH + H+ | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, recombinant N-terminally His6-tagged enzyme, SDS-PAGE | Trypanosoma cruzi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IDH1 | - |
Trypanosoma cruzi |
| NADP-dependent isocitrate dehydrogenase | - |
Trypanosoma cruzi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is completely inactive with NAD+ | Trypanosoma cruzi | |
| NADP+ | - |
Trypanosoma cruzi | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
