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Literature summary for 1.1.1.42 extracted from
- Thermal stability and biochemical properties of isocitrate dehydrogenase from the thermoacidophilic archaeon Thermoplasma acidophilum (2007), Extremophiles, 11, 397-402.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 | Thermoplasma acidophilum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.111 | - |
NADP+ | 60°C, pH 7.5 | Thermoplasma acidophilum |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NADP+ | Thermoplasma acidophilum | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoplasma acidophilum | Q9HLV8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoplasma acidophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NADP+ | - |
Thermoplasma acidophilum | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TaIDH | - |
Thermoplasma acidophilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Thermoplasma acidophilum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 80 | 50°C: about 50% of maximal activity, 80°C: about 65% of maximal activity | Thermoplasma acidophilum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
pH 5, enzyme retains 91% of its activity | Thermoplasma acidophilum |
| 70 | - |
half-life: 88 min | Thermoplasma acidophilum |
| 75 | - |
half-life: 24 min | Thermoplasma acidophilum |
| 82 | - |
melting temperature at pH 7.5 is 82.2°C | Thermoplasma acidophilum |
| 85 | - |
melting temperature at pH 5.8 is 84.5°C | Thermoplasma acidophilum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Thermoplasma acidophilum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 7.5 | three-fold reduction in activity when the pH is shifted from the pH-optimum at 7.5 to pH 5.8 | Thermoplasma acidophilum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
1 h, 60°C, enzyme retains 91% of its activity | Thermoplasma acidophilum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | no activity when NAD+ up to 2 mM | Thermoplasma acidophilum | |
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Release 2023.1 (January 2023)
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