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Literature summary for 1.1.1.42 extracted from

  • Prasad, U.V.; Vasu, D.; Kumar, Y.N.; Kumar, P.S.; Yeswanth, S.; Swarupa, V.; Phaneendra, B.V.; Chaudhary, A.; Sarma, P.V.
    Cloning, expression and characterization of NADP-dependent isocitrate dehydrogenase from Staphylococcus aureus (2013), Appl. Biochem. Biotechnol., 169, 862-869.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene idh, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0082
-
isocitrate recombinant enzyme, pH 7.5, 30°C Staphylococcus aureus
0.0675
-
NADP+ recombinant enzyme, pH 7.5, 30°C Staphylococcus aureus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Staphylococcus aureus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Staphylococcus aureus
-
2-oxoglutarate + CO2 + NADPH + H+
-
?
isocitrate + NADP+ Staphylococcus aureus ATCC 12600
-
2-oxoglutarate + CO2 + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus D7RIE8
-
-
Staphylococcus aureus ATCC 12600 D7RIE8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
Staphylococcus aureus 2-oxoglutarate + CO2 + NADPH + H+
-
?
isocitrate + NADP+
-
Staphylococcus aureus ATCC 12600 2-oxoglutarate + CO2 + NADPH + H+
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Staphylococcus aureus

Synonyms

Synonyms Comment Organism
IDH
-
Staphylococcus aureus
NADP-dependent isocitrate dehydrogenase
-
Staphylococcus aureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on Staphylococcus aureus

General Information

General Information Comment Organism
physiological function isocitrate dehydrogenase plays pivotal role in the growth and pathogenesis of the bacteria Staphylococcus aureus