Cloned (Comment) | Organism |
---|---|
gene idh, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0082 | - |
isocitrate | recombinant enzyme, pH 7.5, 30°C | Staphylococcus aureus | |
0.0675 | - |
NADP+ | recombinant enzyme, pH 7.5, 30°C | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Staphylococcus aureus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Staphylococcus aureus | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | Staphylococcus aureus ATCC 12600 | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | D7RIE8 | - |
- |
Staphylococcus aureus ATCC 12600 | D7RIE8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Staphylococcus aureus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | - |
Staphylococcus aureus ATCC 12600 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
IDH | - |
Staphylococcus aureus |
NADP-dependent isocitrate dehydrogenase | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | dependent on | Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
physiological function | isocitrate dehydrogenase plays pivotal role in the growth and pathogenesis of the bacteria | Staphylococcus aureus |