Literature summary for 1.1.1.42 extracted from

Sidhu, N.S.; Delbaere, L.T.; Sheldrick, G.M.
Structure of a highly NADP+-specific isocitrate dehydrogenase (2011), Acta Crystallogr. Sect. D, 67, 856-869.

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method, 0.001 ml of 10 mg/ml protein in 12.5 mM 2-morpholinoethanesulfonic acid, pH 6.2, with 1.25 mM MnSO4, 1.25 mM dithiothreitol and 10% glycerol, is mixed with 0.001 ml of reservoir solution containing 25% w/v PEG 2000 MME, 0.2 M Tris-HCl, pH 7.3, and 0.2 M MgCl2, and with or without 0.001 ml of 10 mM NADP+, 3 days to 2 months, X-ray diffrcation structure determination and analysis at 1.8-1.9 A resolution, molecular replacement	Corynebacterium glutamicum

Crystallization (Comment)

Organism

hanging drop vapour diffusion method, 0.001 ml of 10 mg/ml protein in 12.5 mM 2-morpholinoethanesulfonic acid, pH 6.2, with 1.25 mM MnSO4, 1.25 mM dithiothreitol and 10% glycerol, is mixed with 0.001 ml of reservoir solution containing 25% w/v PEG 2000 MME, 0.2 M Tris-HCl, pH 7.3, and 0.2 M MgCl2, and with or without 0.001 ml of 10 mM NADP+, 3 days to 2 months, X-ray diffrcation structure determination and analysis at 1.8-1.9 A resolution, molecular replacement

Corynebacterium glutamicum

Inhibitors

Inhibitors	Comment	Organism	Structure
glyoxylate	-	Corynebacterium glutamicum
oxaloacetate	-	Corynebacterium glutamicum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80000	-	1 * 80000	Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
isocitrate + NADP+	Corynebacterium glutamicum	-	2-oxoglutarate + CO2 + NADPH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium glutamicum	P50216	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
isocitrate + NADP+	-	Corynebacterium glutamicum	2-oxoglutarate + CO2 + NADPH + H+	-	?
isocitrate + NADP+	the enzyme shows high substrate specificity	Corynebacterium glutamicum	2-oxoglutarate + CO2 + NADPH + H+	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 80000	Corynebacterium glutamicum

Synonyms

Synonyms	Comment	Organism
NADP+-specific isocitrate dehydrogenase	-	Corynebacterium glutamicum

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	NADP+-binding site in the interdomain interface, the enzyme shows high specificity for NADP+ mediated by interactions with the negatively charged 2'-phosphate group, which is surrounded by the side chains of two arginines, one histidine and, via a water, one lysine residue, forming ion pairs and hydrogen bonds	Corynebacterium glutamicum