Cloned (Comment) | Organism |
---|---|
IDH1, genotyping in acute myelogenous leukemia patients, overview | Homo sapiens |
IDH2, genotyping in acute myelogenous leukemia patients, overview | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R132C | IDH1 R132 mutations cause production and accumulation of 2-hydroxyglutarate in acute myelogenous leukemia cells. The mutation reduces the affinity for isocitrate, and increases the affinity for NADPH and 2-oxoglutarate, preventing the oxidative decarboxylation of isocitrate to 2-oxoglutarate, and facilitating the conversion of 2-oxoglutarate to 2-hydroxyglutarate | Homo sapiens |
R132G | IDH1 R132 mutations cause production and accumulation of 2-hydroxyglutarate in acute myelogenous leukemia cells | Homo sapiens |
R132H | IDH1 R132 mutations cause production and accumulation of 2-hydroxyglutarate in acute myelogenous leukemia cells | Homo sapiens |
R172K | IDH2 R172 mutation causes production and accumulation of 2-hydroxyglutarate in acute myelogenous leukemia cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | IDH1 | Homo sapiens | 5829 | - |
mitochondrion | IDH2 | Homo sapiens | 5739 | - |
peroxisome | IDH1 | Homo sapiens | 5777 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Homo sapiens | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O75874 | IDH1 | - |
Homo sapiens | P48735 | IDH2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
glioblastoma cell | multiforme tumors | Homo sapiens | - |
glioma cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Homo sapiens | 2-oxoglutarate + NADPH + H+ + CO2 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
IDH1 | - |
Homo sapiens |
IDH2 | - |
Homo sapiens |
isocitrate dehydrogenase 1 | - |
Homo sapiens |
isocitrate dehydrogenase 2 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | IDH1 is critical in cellular metabolism | Homo sapiens |
physiological function | mutation at R172 in the active site of IDH2 leads to a change in the molecular mechanism of enzyme catalysis, resulting in production and accumulation of elevated 2-hydroxyglutarate in acute myelogenous leukemia. The mutation reduces the affinity for isocitrate, and increases the affinity for NADPH and 2-oxoglutarate, preventing the oxidative decarboxylation of isocitrate to 2-oxoglutarate, and facilitating the conversion of 2-oxoglutarate to 2-hydroxyglutarate | Homo sapiens |
physiological function | mutations at R132 in the active site of IDH1 lead to a change in the molecular mechanism of enzyme catalysis, resulting in production and accumulation of elevated 2-hydroxyglutarate in acute myelogenous leukemia. The mutations reduce the affinity for isocitrate, and increase the affinity for NADPH and 2-oxoglutarate, preventing the oxidative decarboxylation of isocitrate to 2-oxoglutarate, and facilitating the conversion of 2-oxoglutarate to 2-hydroxyglutarate | Homo sapiens |