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Literature summary for 1.1.1.42 extracted from

  • Shin, S.W.; Kil, I.S.; Park, J.W.
    Cytosolic NADP(+)-dependent isocitrate dehydrogenase regulates cadmium-induced apoptosis (2010), Biochem. Pharmacol., 79, 1072-1080.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged mutant enzymes C269S and C379S in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
C269S site-directed mutagenesis, is not sensitive to inhibition by Cd2+ in contrast to the wild-type enzyme Homo sapiens
C379S site-directed mutagenesis, is inhibited by Cd2+ in a similar manner to the wild-type Homo sapiens
additional information transfection of HEK-293 cells with an IDPc small interfering RNA significantly decreases the activity of IDPc and enhances cellular susceptibility to cadmium-induced apoptosis as indicated by the morphological evidence of apoptosis, DNA fragmentation and condensation, cellular redox status, mitochondria redox status and function, and the modulation of apoptotic marker proteins, overview. DNA fragmentation is enhanced in IDPc siRNA-transfected HEK293 cells compared to control cells upon exposure to cadmium Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ inhibits the purified enzyme and the enzyme in cells, NADP+ does not protect. No inhibition of IDPc mutant S269S. DNA fragmentation is enhanced in IDPc siRNA-transfected HEK293 cells compared to control cells upon exposure to cadmium Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic isozyme IDPc, subcellular localization study, overview Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Homo sapiens
-
2-oxoglutarate + NADPH + H+ + CO2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged mutant enzymes C269S and C379S from Escherichia coli by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
Homo sapiens 2-oxoglutarate + NADPH + H+ + CO2
-
?

Synonyms

Synonyms Comment Organism
cytosolic NADP(+)-dependent isocitrate dehydrogenase
-
Homo sapiens
IDPc
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on Homo sapiens

General Information

General Information Comment Organism
additional information knockdown of IDPc expression in HEK293 cells greatly enhances apoptosis induced by cadmium. DNA fragmentation is enhanced in IDPc siRNA-transfected HEK293 cells compared to control cells upon exposure to cadmium Homo sapiens