Cloned (Comment) | Organism |
---|---|
expression of His-tagged IDPA in Escherichia coli strain BL21(DE3), expression of IDPA inm Saccharomyces cerevisiae, it behaves similar to the yeast IDP2 harboring a type I peroxisomal targeting sequenceand occurs in cytosol and peroxisomes, complementation study in Saccharomyces cerevisiae disruption mutants expressing the full-length IDPA enzyme from Aspergillus nidulans, and IDPA lacking the mitochondrial targeting sequence and containing a different PTS1, which results in the same expression level and subcellular orientation, subcellular localization study, overview | Aspergillus nidulans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of IDP1, IDP2, and IDP3 disruption mutants, and of double and triple disruption mutants in haploid strain MMY011. Complementation study in disruption mutants expressing the full-length IDPA enzyme from Aspergillus nidulans, which behaves similar to the yeast IDP2 harboring a type I peroxisomal targeting sequence, PTS1, and occurs in cytosol and peroxisomes, subcellular localization study, overview. Expression of IDPA lacking the mitochondrial targeting sequence and containing a different PTS1 results in the same expression level and subcellular orientation | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Aspergillus nidulans | 5829 | - |
cytosol | IDP2 carrying a type I peroxisomal targeting sequence, IDP2+CKL, is only partially localized to peroxisomes, the enzyme is able to function either instead of peroxisomal IDP3 or as cytosolic IDP2 | Saccharomyces cerevisiae | 5829 | - |
mitochondrion | IDP1 | Saccharomyces cerevisiae | 5739 | - |
additional information | subcellular localization study of isozymes, overview | Saccharomyces cerevisiae | - |
- |
peroxisome | - |
Aspergillus nidulans | 5777 | - |
peroxisome | IDP3 contains a canonical type I peroxisomal targeting sequence, a carboxyl-terminal Cys-Lys-Leu tripeptide. IDP3 is normally strictly peroxisomal | Saccharomyces cerevisiae | 5777 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Saccharomyces cerevisiae | |
Mg2+ | activates | Aspergillus nidulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Saccharomyces cerevisiae | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
isocitrate + NADP+ | Aspergillus nidulans | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
isocitrate + NADP+ | Saccharomyces cerevisiae MMY011 | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae MMY011 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged IDPA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Aspergillus nidulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Saccharomyces cerevisiae | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
isocitrate + NADP+ | - |
Aspergillus nidulans | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
isocitrate + NADP+ | - |
Saccharomyces cerevisiae MMY011 | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDP3 | - |
Saccharomyces cerevisiae |
IDPA | - |
Aspergillus nidulans |
NADP+-specific isocitrate dehydrogenase | - |
Saccharomyces cerevisiae |
NADP+-specific isocitrate dehydrogenase | - |
Aspergillus nidulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
IDP2 | Saccharomyces cerevisiae |
8 | - |
- |
Aspergillus nidulans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | 80% of maximal activity in the pH range of pH 7.0-9.0 | Aspergillus nidulans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | specific for | Saccharomyces cerevisiae | |
NADP+ | specific for | Aspergillus nidulans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharomyces cerevisiae | IDP2, isoelectric focusing | - |
6.5 |
Aspergillus nidulans | IDPA, isoelectric focusing | - |
7.4 |
Saccharomyces cerevisiae | IDP1, isoelectric focusing | - |
8.2 |
Saccharomyces cerevisiae | IDP3, isoelectric focusing | - |
8.9 |
General Information | Comment | Organism |
---|---|---|
physiological function | IDP3 provides the NADPH required for beta-oxidation of some fatty acids in the peroxisome | Saccharomyces cerevisiae |