Literature summary for 1.1.1.42 extracted from

Fedoy, A.E.; Yang, N.; Martinez, A.; Leiros, H.K.; Steen, I.H.
Structural and functional properties of isocitrate dehydrogenase from the psychrophilic bacterium Desulfotalea psychrophila reveal a cold-active enzyme with an unusual high thermal stability (2007), J. Mol. Biol., 372, 130-149.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of native DpIDH are obtained by the sitting-drop, vapour-diffusion method. The crystals are grown at 8°C. Crystals of DpIDH in complex with isocitrate (DpIDH-iso) are obtained by the hanging drop method at 8 °C	Desulfotalea psychrophila

Organism

Organism	UniProt	Comment	Textmining
Desulfotalea psychrophila	Q6AQ66	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
isocitrate + NADP+	-	Desulfotalea psychrophila	2-oxoglutarate + CO2 + NADPH + H+	-	?

Synonyms

Synonyms	Comment	Organism
DpIDH	-	Desulfotalea psychrophila

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	-	melting temperature is 66.9°C	Desulfotalea psychrophila

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.5	-	NADP+	5°C	Desulfotalea psychrophila
4.5	-	isocitrate	5°C	Desulfotalea psychrophila
31.4	-	NADP+	25°C	Desulfotalea psychrophila
31.4	-	isocitrate	25°C	Desulfotalea psychrophila

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Release 2023.1 (January 2023)