Protein Variants | Comment | Organism |
---|---|---|
R291S | involved in coenzyme specificity | Haloferax volcanii |
R291S/K343D/Y344I/V350A/Y390P | switch in coenzyme specificity from NADP+ to NAD+ | Haloferax volcanii |
R291S/K343D/Y344I/Y390P | involved in coenzyme specificity | Haloferax volcanii |
R291S/K343D/Y390P | involved in coenzyme specificity | Haloferax volcanii |
R291S/Y390P | involved in coenzyme specificity | Haloferax volcanii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
DL-isocitrate | mutant R291S/K343D/Y344I/V350A/Y390P, pH 8.0, 30°C | Haloferax volcanii | |
0.018 | - |
DL-isocitrate | mutant R291S, pH 8.0, 30°C | Haloferax volcanii | |
0.036 | - |
DL-isocitrate | mutant R291S/K343D/Y390P, pH 8.0, 30°C | Haloferax volcanii | |
0.036 | - |
DL-isocitrate | mutant R291S/Y390P, pH 8.0, 30°C | Haloferax volcanii | |
0.04 | - |
DL-isocitrate | mutant R291S/K343D/Y344I/Y390P, pH 8.0, 30°C | Haloferax volcanii | |
0.101 | - |
NADP+ | wild-type, pH 8.0, 30°C | Haloferax volcanii | |
0.108 | - |
DL-isocitrate | wild-type, pH 8.0, 30°C | Haloferax volcanii | |
0.144 | - |
NAD+ | mutant R291S/K343D/Y344I/V350A/Y390P, pH 8.0, 30°C | Haloferax volcanii | |
0.203 | - |
NADP+ | mutant R291S/K343D/Y390P, pH 8.0, 30°C | Haloferax volcanii | |
0.282 | - |
NADP+ | mutant R291S/K343D/Y344I/Y390P, pH 8.0, 30°C | Haloferax volcanii | |
0.327 | - |
NADP+ | mutant R291S/Y390P, pH 8.0, 30°C | Haloferax volcanii | |
0.35 | - |
NADP+ | mutant R291S, pH 8.0, 30°C | Haloferax volcanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax volcanii | Q8X277 | recombinant enzyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-isocitrate + NAD+ | NAD+ is only substrate for mutant R291S/K343D/Y344I/V350A/Y390P | Haloferax volcanii | 2-oxoglutarate + CO2 + NADH | - |
? | |
DL-isocitrate + NADP+ | NAD+ is not substrate for mutant R291S/K343D/Y344I/V350A/Y390P | Haloferax volcanii | 2-oxoglutarate + CO2 + NADPH | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
NADP+ | mutant R291S/K343D/Y344I/Y390P, pH 8.0, 30°C | Haloferax volcanii | |
0.0005 | - |
DL-isocitrate | mutant R291S/Y390P, pH 8.0, 30°C | Haloferax volcanii | |
0.0007 | - |
NAD+ | mutant R291S/K343D/Y344I/V350A/Y390P, pH 8.0, 30°C | Haloferax volcanii | |
0.001 | - |
NADP+ | mutant R291S/Y390P, pH 8.0, 30°C | Haloferax volcanii | |
0.0013 | - |
DL-isocitrate | mutant R291S/K343D/Y390P, pH 8.0, 30°C | Haloferax volcanii | |
0.002 | - |
DL-isocitrate | mutant R291S, pH 8.0, 30°C | Haloferax volcanii | |
0.0023 | - |
DL-isocitrate | wild-type, pH 8.0, 30°C | Haloferax volcanii | |
0.0024 | - |
NADP+ | mutant R291S/K343D/Y390P, pH 8.0, 30°C | Haloferax volcanii | |
0.0025 | - |
DL-isocitrate | mutant R291S/K343D/Y344I/Y390P, pH 8.0, 30°C | Haloferax volcanii | |
0.003 | - |
NADP+ | wild-type, pH 8.0, 30°C | Haloferax volcanii | |
0.004 | - |
NADP+ | mutant R291S, pH 8.0, 30°C | Haloferax volcanii | |
0.005 | - |
DL-isocitrate | mutant R291S/K343D/Y344I/V350A/Y390P, pH 8.0, 30°C | Haloferax volcanii |