Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | glucose induces epression of isozyme IDP1, glycerol induces epression of isozymes IDP1 and IDP2, fatty acids induce epression of isozymes IDP1, IDP2, and IDP3 | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression of C-terminally His-tagged enzyme in Escherichia coli BL21(DE3) | Homo sapiens |
expression of isozyme IDP1 and IDP2 as His-tagged enzymes in a disruption mutant | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, purified recombinant His-tagged enzyme in complex with NADP+: equal volume of protein solution, containing 15 mg/ml enzyme, 20 mM Tris-HCl, pH 7.4, 100 mM NaCl, and 10 mM NADP+, and of reservoir solution, containing 100 mM MES, pH 6.5, 12% PEG 20000, at 4°C, purified recombinant His-tagged enzyme in complex with NADP+, isocitrate and Ca2+: equal volume of protein solution, containing 15 mg/ml enzyme, 20 mM Tris-HCl, pH 7.4, 100 mM NaCl, and 10 mM NADP+, 10 mM DL-isocitrate and 10 mM CaCl2, and of reservoir solution, containing 100 mM MES, pH 5.9, 20% PEG 6000, at 20°C, X-ray diffraction structure determination and analysis | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of isozymes IDP1 and IDP2 | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | isozyme IDP2 | Saccharomyces cerevisiae | 5829 | - |
mitochondrion | isozyme IDP1 | Saccharomyces cerevisiae | 5739 | - |
peroxisome | isoyzme IDP3 | Saccharomyces cerevisiae | 5777 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | divalent metal ion is required, binding structure and conformation at the isocitrate-metal-binding site | Homo sapiens | |
Mg2+ | - |
Saccharomyces cerevisiae | |
Mg2+ | divalent metal ion is required, binding structure and conformation at the isocitrate-metal-binding site | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46381 | - |
x * 46381, isozyme IDP1, mass spectrometry, x * 46562, isozyme IDP2, mass spectrometry, x * 47856, isozyme IDP3, mass spectrometry | Saccharomyces cerevisiae |
46562 | - |
x * 46381, isozyme IDP1, mass spectrometry, x * 46562, isozyme IDP2, mass spectrometry, x * 47856, isozyme IDP3, mass spectrometry | Saccharomyces cerevisiae |
47856 | - |
x * 46381, isozyme IDP1, mass spectrometry, x * 46562, isozyme IDP2, mass spectrometry, x * 47856, isozyme IDP3, mass spectrometry | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-isocitrate + NADP+ | Saccharomyces cerevisiae | the reaction is reversible for isozyme IDP2 | 2-oxoglutarate + CO2 + NADPH | - |
r | |
isocitrate + NADP+ | Homo sapiens | enzyme has a self-regulatory mechanism of activity mimicking the phosphorylation mechanism of bacterial enzymes | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Saccharomyces cerevisiae | - |
1 cytosolic, 1 peroxisomal, and 1 mitochondrial isozyme | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Homo sapiens |
recombinant His-tagged isozymes IDP1 and IDP2 by nickel affinity chromatography | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH + H+ | active site structure and reaction mechanism, conformational changes at the active site resulting in closed and open forms, regulatory residues are isocitrate-binding Asp279and Ser94 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-isocitrate + NADP+ | the reaction is reversible for isozyme IDP2 | Saccharomyces cerevisiae | 2-oxoglutarate + CO2 + NADPH | - |
r | |
isocitrate + NADP+ | - |
Homo sapiens | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | enzyme has a self-regulatory mechanism of activity mimicking the phosphorylation mechanism of bacterial enzymes | Homo sapiens | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46381, isozyme IDP1, mass spectrometry, x * 46562, isozyme IDP2, mass spectrometry, x * 47856, isozyme IDP3, mass spectrometry | Saccharomyces cerevisiae |
More | enzyme has 3 different conformational stages | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
IDP | - |
Saccharomyces cerevisiae |
NADP-dependent isocitrate dehydrogenase | - |
Homo sapiens |
NADP-dependent isocitrate dehydrogenase | - |
Saccharomyces cerevisiae |
NADP-IDH | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at, reverse reaction | Saccharomyces cerevisiae |
7.4 | - |
assay at | Homo sapiens |
7.5 | - |
isozyme IDP2, forward reaction | Saccharomyces cerevisiae |
8 | - |
isozyme IDP1, forward reaction | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Saccharomyces cerevisiae | |
NADP+ | binding site structure, interaction with the 2'-phosphate group of the ribose moiety | Homo sapiens | |
NADPH | - |
Saccharomyces cerevisiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharomyces cerevisiae | isozyme IDP2 | - |
5.5 |
Saccharomyces cerevisiae | isozyme IDP1 | - |
8.5 |
Saccharomyces cerevisiae | isozyme IDP3 | - |
10 |