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Literature summary for 1.1.1.42 extracted from
- Regulation of NAD- and NADP-dependent isocitrate dehydrogenases by reduction levels of pyridine nucleotides in mitochondria and cytosol of pea leaves (2003), Biochim. Biophys. Acta, 1606, 117-125.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the mitochondrial isozyme is light-inducible, while the cytosolic one is not | Pisum sativum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | - |
Pisum sativum |  |
| isocitrate | reverse reaction | Pisum sativum | |
| additional information | poor inhibition of the forward reaction by 2-oxoglutarate, no inhibition by CO2 | Pisum sativum | |
| NADH | noncompetitive | Pisum sativum | |
| NADP+ | reverse reaction | Pisum sativum | |
| NADPH | forward reaction | Pisum sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
NADP+ | mitochondrial isozyme, forward reaction, pH 8.0 | Pisum sativum | |
| 0.004 | - |
NADP+ | cytosolic isozyme, forward reaction, pH 8.0 | Pisum sativum | |
| 0.004 | - |
isocitrate | mitochondrial isozyme, forward reaction, pH 8.0 | Pisum sativum | |
| 0.006 | - |
isocitrate | cytosolic isozyme, forward reaction, pH 8.0 | Pisum sativum | |
| 0.009 | - |
NADPH | mitochondrial isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
| 0.017 | - |
2-oxoglutarate | mitochondrial isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
| 0.024 | - |
NADPH | cytosolic isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
| 0.026 | - |
2-oxoglutarate | cytosolic isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
| 0.2 | - |
CO2 | mitochondrial isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
| 1.6 | - |
CO2 | cytosolic isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Pisum sativum | 5829 | - |
| mitochondrion | light-inducible isozyme | Pisum sativum | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NADP+ | Pisum sativum | the cytosolic isozyme supplies 2-oxoglutarate for the photorespiratory ammonia fixation, pyridine nucleotide contents in mitochondria and cytosol, regulation, overview | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
cv. 294 040 Märgärt Kelvedon Wonder, one cytosolic and one mitochondrial isozyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cytosolic and mitochondrial isozymes | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | green and etiolated | Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.038 | - |
mitochondria from etiolated leaves, forward reaction at pH 7.5 | Pisum sativum |
| 0.072 | - |
mitochondria from green leaves, forward reaction at pH 7.5 | Pisum sativum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, partially purified cytosolic and mitochondrial isozymes, several months, stable | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NADP+ | - |
Pisum sativum | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
| isocitrate + NADP+ | the cytosolic isozyme supplies 2-oxoglutarate for the photorespiratory ammonia fixation, pyridine nucleotide contents in mitochondria and cytosol, regulation, overview | Pisum sativum | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADP-dependent isocitrate dehydrogenase | - |
Pisum sativum |
| NADP-ICDH | - |
Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 6.5 | reverse reaction, cytosolic isozyme | Pisum sativum |
| 6.8 | 7 | reverse reaction, mitochondrial isozyme | Pisum sativum |
| 8 | 9 | forward reaction, cytosolic and mitochondrial isozymes | Pisum sativum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with NAD+ and NADH | Pisum sativum | |
| NADP+ | absolutely specific for, forward reaction | Pisum sativum | |
| NADPH | absolutely specific for, reverse reaction | Pisum sativum | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
NADPH | mitochondrial isozyme, forward reaction, pH 8.0 | Pisum sativum | |
| 0.005 | - |
isocitrate | mitochondrial isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
| 0.006 | - |
NADP+ | mitochondrial isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
| 0.007 | - |
NADPH | cytosolic isozyme, forward reaction, pH 8.0 | Pisum sativum | |
| 0.012 | - |
isocitrate | cytosolic isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
| 0.021 | - |
NADP+ | cytosolic isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
| 0.06 | - |
NADH | mitochondrial isozyme, forward reaction, pH 8.0 | Pisum sativum | |
| 0.08 | - |
NADH | cytosolic isozyme, forward reaction, pH 8.0 | Pisum sativum | |
| 1 | - |
2-oxoglutarate | above, cytosolic isozyme, forward reaction, pH 8.0 | Pisum sativum | |
| 1 | - |
2-oxoglutarate | above, mitochondrial isozyme, forward reaction, pH 8.0 | Pisum sativum | |
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