KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
NADP+ | Mg2+-dependent reaction, ICDH2 | Nicotiana tabacum | |
0.0075 | - |
NADP+ | Mg2+-dependent reaction, ICDH1 | Nicotiana tabacum | |
0.012 | - |
Mg2+ | Mg2+-dependent reaction, ICDH2 | Nicotiana tabacum | |
0.013 | - |
Mg2+ | Mg2+-dependent reaction, ICDH1 | Nicotiana tabacum | |
0.028 | - |
D-isocitrate | Mg2+-dependent reaction, ICDH2 | Nicotiana tabacum | |
0.028 | - |
D-isocitrate | chloroplastic enzyme, at pH 7.5 and 30°C | Nicotiana tabacum | |
0.041 | - |
D-isocitrate | Mg2+-dependent reaction, ICDH1 | Nicotiana tabacum | |
0.041 | - |
D-isocitrate | cytosolic enzyme, at pH 7.5 and 30°C | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | ICDH2 | Nicotiana tabacum | 9507 | - |
cytosol | ICDH1 | Nicotiana tabacum | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
117000 | - |
gel filtration, ICDH1 | Nicotiana tabacum |
136000 | - |
gel filtration, ICDH2 | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Nicotiana tabacum | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Nicotiana tabacum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.6 | - |
ICDH2 | Nicotiana tabacum |
50 | - |
ICDH1 | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-isocitrate + NADP+ | - |
Nicotiana tabacum | 2-oxoglutarate + CO2 + NADPH | - |
r | |
isocitrate + NADP+ | - |
Nicotiana tabacum | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Nicotiana tabacum |