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Literature summary for 1.1.1.42 extracted from

  • Galvez, S.; Bismuth, E.; Sarda, C.; Gadal, P.
    Purification and characterization of chloroplastic NADP-isocitrate dehydrogenase from mixotrophic tobacco cells (1994), Plant Physiol., 105, 593-600.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
NADP+ Mg2+-dependent reaction, ICDH2 Nicotiana tabacum
0.0075
-
NADP+ Mg2+-dependent reaction, ICDH1 Nicotiana tabacum
0.012
-
Mg2+ Mg2+-dependent reaction, ICDH2 Nicotiana tabacum
0.013
-
Mg2+ Mg2+-dependent reaction, ICDH1 Nicotiana tabacum
0.028
-
D-isocitrate Mg2+-dependent reaction, ICDH2 Nicotiana tabacum
0.028
-
D-isocitrate chloroplastic enzyme, at pH 7.5 and 30°C Nicotiana tabacum
0.041
-
D-isocitrate Mg2+-dependent reaction, ICDH1 Nicotiana tabacum
0.041
-
D-isocitrate cytosolic enzyme, at pH 7.5 and 30°C Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast ICDH2 Nicotiana tabacum 9507
-
cytosol ICDH1 Nicotiana tabacum 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
117000
-
gel filtration, ICDH1 Nicotiana tabacum
136000
-
gel filtration, ICDH2 Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Nicotiana tabacum
-
2-oxoglutarate + NADPH + H+ + CO2
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.6
-
ICDH2 Nicotiana tabacum
50
-
ICDH1 Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-isocitrate + NADP+
-
Nicotiana tabacum 2-oxoglutarate + CO2 + NADPH
-
r
isocitrate + NADP+
-
Nicotiana tabacum 2-oxoglutarate + NADPH + H+ + CO2
-
?

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Nicotiana tabacum