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Literature summary for 1.1.1.42 extracted from

  • Curry, R.A.; Ting, I.P.
    Purification, properties, and kinetic observations on the isoenzymes of NADP isocitrate dehydrogenase of maize (1976), Arch. Biochem. Biophys., 176, 501-509.
    View publication on PubMed

General Stability

General Stability Organism
instable in solutions of low ionic strength, stabilization by ammonium sulfate Sus scrofa
instable in solutions of low ionic strength, stabilization by ammonium sulfate Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
D,L-isocitrate isozyme 2 Zea mays
0.003
-
NADP+ isozyme 2 Zea mays
0.0031
-
D,L-isocitrate isozyme 1 Zea mays
0.0063
-
NADP+ isozyme 1 Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Sus scrofa 5739
-
mitochondrion
-
Geobacillus stearothermophilus 5739
-
mitochondrion
-
Zea mays 5739
-
mitochondrion
-
Azotobacter sp. 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
gel filtration Sus scrofa
81000 83000 gel filtration Zea mays
105000
-
gel filtration Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Sus scrofa
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+ Geobacillus stearothermophilus
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+ Cereibacter sphaeroides
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+ Zea mays
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+ Azotobacter sp.
-
2-oxoglutarate + NADPH + H+ + CO2
-
?

Organism

Organism UniProt Comment Textmining
Azotobacter sp.
-
-
-
Cereibacter sphaeroides
-
i.e. Rhodobacter sphaeroides
-
Geobacillus stearothermophilus
-
-
-
Sus scrofa
-
pig
-
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Zea mays

Renatured (Commentary)

Renatured (Comment) Organism
overnight dialysis against guanidine-HCl, GDP buffer Sus scrofa
overnight dialysis against guanidine-HCl, GDP buffer Geobacillus stearothermophilus
overnight dialysis against guanidine-HCl, GDP buffer Cereibacter sphaeroides
overnight dialysis against guanidine-HCl, GDP buffer Zea mays
overnight dialysis against guanidine-HCl, GDP buffer Azotobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Sus scrofa
-
seed
-
Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33.5
-
-
Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
Sus scrofa 2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+
-
Geobacillus stearothermophilus 2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+
-
Cereibacter sphaeroides 2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+
-
Zea mays 2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+
-
Azotobacter sp. 2-oxoglutarate + NADPH + H+ + CO2
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Zea mays