Cloned (Comment) | Organism |
---|---|
gene idh, expression of wild-type and mutant enzymes in Escherichia coli glutamate auxotrophic strain Dicd::kanr fused to gene icd, the endogenous icd gene is replaced by the kanamycin resistance gene, the wild-type phenotype of the icd defective strain on minimal medium without glutamate is restored | Streptococcus mutans |
Protein Variants | Comment | Organism |
---|---|---|
S102A | site-directed mutagenesis, the mutant shows decreased affinity for isocitrate and 3.3% of wild-type activity | Streptococcus mutans |
S102G | site-directed mutagenesis, the mutant shows decreased affinity for isocitrate and 2.8% of wild-type activity | Streptococcus mutans |
S102T | site-directed mutagenesis, the mutant shows decreased affinity for isocitrate and 16% of wild-type activity | Streptococcus mutans |
S102Y | site-directed mutagenesis, the mutant shows decreased affinity for isocitrate and 1.1% of wild-type activity | Streptococcus mutans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Streptococcus mutans | |
Co2+ | - |
Streptococcus mutans | |
Cu2+ | - |
Streptococcus mutans | |
Ni2+ | - |
Streptococcus mutans | |
Zn2+ | - |
Streptococcus mutans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
DL-isocitrate | pH 7.5, 37°C, recombinant wild-type enzyme | Streptococcus mutans | |
0.143 | - |
DL-isocitrate | pH 7.5, 37°C, recombinant enzyme mutant S102G | Streptococcus mutans | |
0.148 | - |
DL-isocitrate | pH 7.5, 37°C, recombinant enzyme mutant S102A | Streptococcus mutans | |
0.15 | - |
DL-isocitrate | pH 7.5, 37°C, recombinant enzyme mutant S102T | Streptococcus mutans | |
0.154 | - |
NAD+ | pH 7.5, 37°C, recombinant enzyme | Streptococcus mutans | |
0.246 | - |
NAD+ | pH 7.5, 37°C, recombinant enzyme mutant S102A | Streptococcus mutans | |
0.295 | - |
NAD+ | pH 7.5, 37°C, recombinant enzyme mutant S102G | Streptococcus mutans | |
0.35 | - |
NAD+ | pH 7.5, 37°C, recombinant enzyme mutant S102T | Streptococcus mutans | |
0.385 | - |
DL-isocitrate | pH 7.5, 37°C, recombinant enzyme mutant S102Y | Streptococcus mutans | |
1.56 | - |
NAD+ | pH 7.5, 37°C, recombinant enzyme mutant S102Y | Streptococcus mutans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | best divalent cation | Streptococcus mutans | |
additional information | the enzyme is dependent on divalent cations | Streptococcus mutans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
recombinant enzyme, gel filtration | Streptococcus mutans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-isocitrate + NAD+ | Streptococcus mutans | - |
2-oxoglutarate + CO2 + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | catalytically important phosphorylation site at Ser102 | Streptococcus mutans |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli | Streptococcus mutans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-isocitrate + NAD+ | - |
Streptococcus mutans | 2-oxoglutarate + CO2 + NADH | - |
r | |
additional information | the enzyme is also active with NADP+ and NADPH, cf. EC 1.1.1.42 | Streptococcus mutans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Streptococcus mutans |
Synonyms | Comment | Organism |
---|---|---|
IDH | - |
Streptococcus mutans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Streptococcus mutans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | activity range, profile overview | Streptococcus mutans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant enzyme, stable up to, rapid inactivation above | Streptococcus mutans |
50 | - |
purified recombinant enzyme, inactivation | Streptococcus mutans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
DL-isocitrate | pH 7.5, 37°C, recombinant enzyme mutant S102Y | Streptococcus mutans | |
3 | - |
NAD+ | pH 7.5, 37°C, recombinant enzyme mutant S102A | Streptococcus mutans | |
3.3 | - |
DL-isocitrate | pH 7.5, 37°C, recombinant enzyme mutant S102A | Streptococcus mutans | |
3.5 | - |
NAD+ | pH 7.5, 37°C, recombinant enzyme mutant S102Y | Streptococcus mutans | |
4 | - |
DL-isocitrate | pH 7.5, 37°C, recombinant enzyme mutant S102G | Streptococcus mutans | |
9.1 | - |
NAD+ | pH 7.5, 37°C, recombinant enzyme mutant S102G | Streptococcus mutans | |
25 | - |
DL-isocitrate | pH 7.5, 37°C, recombinant enzyme mutant S102T | Streptococcus mutans | |
56 | - |
NAD+ | pH 7.5, 37°C, recombinant wild-type enzyme | Streptococcus mutans | |
59 | - |
NAD+ | pH 7.5, 37°C, recombinant enzyme mutant S102T | Streptococcus mutans | |
124 | - |
DL-isocitrate | pH 7.5, 37°C, recombinant wild-type enzyme | Streptococcus mutans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Streptococcus mutans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 9.2 | activity range, profile overview | Streptococcus mutans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is also active with NADP+ and NADPH, cf. EC 1.1.1.42 | Streptococcus mutans | |
NAD+ | - |
Streptococcus mutans | |
NADH | - |
Streptococcus mutans |
General Information | Comment | Organism |
---|---|---|
evolution | the ancient NAD-dependent IDHs might be the underlying origin of phosphorylation mechanism used by their bacterial NADP-dependent homologues | Streptococcus mutans |
additional information | Ser102 plays an important role in substrate binding and is required for the enzyme function | Streptococcus mutans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000002 | - |
NAD+ | pH 7.5, 37°C, recombinant enzyme mutant S102Y | Streptococcus mutans | |
0.000004 | - |
DL-isocitrate | pH 7.5, 37°C, recombinant enzyme mutant S102Y | Streptococcus mutans | |
0.00001 | - |
NAD+ | pH 7.5, 37°C, recombinant enzyme mutant S102A | Streptococcus mutans | |
0.000022 | - |
DL-isocitrate | pH 7.5, 37°C, recombinant enzyme mutant S102A | Streptococcus mutans | |
0.000028 | - |
DL-isocitrate | pH 7.5, 37°C, recombinant enzyme mutant S102G | Streptococcus mutans | |
0.00003 | - |
NAD+ | pH 7.5, 37°C, recombinant enzyme mutant S102G | Streptococcus mutans | |
0.00017 | - |
NAD+ | pH 7.5, 37°C, recombinant enzyme mutant S102T | Streptococcus mutans | |
0.00017 | - |
DL-isocitrate | pH 7.5, 37°C, recombinant enzyme mutant S102T | Streptococcus mutans | |
0.00036 | - |
NAD+ | pH 7.5, 37°C, recombinant enzyme | Streptococcus mutans | |
0.00165 | - |
DL-isocitrate | pH 7.5, 37°C, recombinant wild-type enzyme | Streptococcus mutans |