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Literature summary for 1.1.1.41 extracted from

  • Watanabe, S.; Yasutake, Y.; Tanaka, I.; Takada, Y.
    Elucidation of stability determinants of cold-adapted monomeric isocitrate dehydrogenase from a psychrophilic bacterium, Colwellia maris, by construction of chimeric enzymes (2005), Microbiology, 151, 1083-1094.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Azotobacter vinelandii
expressed in Escherichia coli Colwellia maris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.8
-
NADP+
-
Azotobacter vinelandii
6.9
-
NADP+
-
Colwellia maris
7.9
-
D-isocitrate
-
Azotobacter vinelandii
62.2
-
D-isocitrate
-
Colwellia maris

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Azotobacter vinelandii
Mg2+
-
Colwellia maris
Mn2+
-
Azotobacter vinelandii
Mn2+
-
Colwellia maris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
1 * 80000, SDS-PAGE Azotobacter vinelandii
80000
-
1 * 80000, SDS-PAGE Colwellia maris

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-
Colwellia maris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-affinity chromatography Azotobacter vinelandii
Ni2+-affinity chromatography Colwellia maris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50.5
-
with 0.12 mM NADP+ and 2 mM D-isocitrate Colwellia maris
75.5
-
with 0.12 mM NADP+ and 2 mM D-isocitrate Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-isocitrate + NAD+
-
Azotobacter vinelandii 2-oxoglutarate + CO2 + NADH
-
r
D-isocitrate + NAD+
-
Colwellia maris 2-oxoglutarate + CO2 + NADH
-
r
isocitrate + NADP+
-
Azotobacter vinelandii 2-oxoglutarate + CO2 + NADPH + H+
-
r
isocitrate + NADP+
-
Colwellia maris 2-oxoglutarate + CO2 + NADPH + H+
-
r

Subunits

Subunits Comment Organism
monomer 1 * 80000, SDS-PAGE Azotobacter vinelandii
monomer 1 * 80000, SDS-PAGE Colwellia maris

Synonyms

Synonyms Comment Organism
IDH
-
Azotobacter vinelandii
IDH
-
Colwellia maris
isocitrate dehydrogenase
-
Azotobacter vinelandii
isocitrate dehydrogenase
-
Colwellia maris
NAD(P)+-dependent isocitrate dehydrogenase
-
Azotobacter vinelandii
NAD(P)+-dependent isocitrate dehydrogenase
-
Colwellia maris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Colwellia maris
47.5
-
-
Azotobacter vinelandii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
57.5
-
D-isocitrate
-
Colwellia maris
61.3
-
NADP+
-
Colwellia maris
73
-
D-isocitrate
-
Azotobacter vinelandii
92.6
-
NADP+
-
Azotobacter vinelandii

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Azotobacter vinelandii
NADP+
-
Colwellia maris