Cloned (Comment) | Organism |
---|---|
gene idh-II | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a knockout mutant of subunit IDH-II, disruption by dissociation insertion element, mutant strain shows normal growth and development, but reduced enzyme activity in mitochondria compared to the wild-type plants, no altered phenotype of the mutant plants, but slightly reduced growth | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | Arabidopsis thaliana | key enzyme in the Krebs cycle | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NAD+ | Arabidopsis thaliana Landsberg | key enzyme in the Krebs cycle | 2-oxoglutarate + CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
Landsberg | - |
Arabidopsis thaliana Landsberg | - |
Landsberg | - |
Purification (Comment) | Organism |
---|---|
mitochondrial preparation | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
plant | from liquid shaker cultures | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
silique | young | Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.092 | - |
partially purified enzyme, idh-II mutant | Arabidopsis thaliana |
0.169 | - |
partially purified enzyme, wild-type | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | key enzyme in the Krebs cycle | Arabidopsis thaliana | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NAD+ | subunit IDH-I can substitute for a deficient mutant subunit IDH-II | Arabidopsis thaliana | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NAD+ | key enzyme in the Krebs cycle | Arabidopsis thaliana Landsberg | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NAD+ | subunit IDH-I can substitute for a deficient mutant subunit IDH-II | Arabidopsis thaliana Landsberg | 2-oxoglutarate + CO2 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | subunit IDH-I can substitute for a deficient mutant subunit IDH-II | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
IDH | - |
Arabidopsis thaliana |
NAD+-dependent isocitrate dehydrogenase | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Arabidopsis thaliana |