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Literature summary for 1.1.1.399 extracted from

  • Zhao, G.; Winkler, M.E.
    A novel alpha-ketoglutarate reductase activity of the serA-encoded 3-phosphoglycerate dehydrogenase of Escherichia coli K-12, and its possible implications for human 2-hydroxyglutaric aciduria (1996), J. Bacteriol., 178, 232-239.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM105 cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
3-phosphoglycerate noncompetitive inhibition Escherichia coli
D-2-hydroxyglutaric acid competitive inhibition Escherichia coli
glycine
-
 Escherichia coli
L-serine noncompetitive inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.088
-
 2-oxoglutarate at pH 7.5 and 37°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 x * 46000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-2-hydroxyglutarate + NAD+ Escherichia coli
-
 2-oxoglutarate + NADH + H+
-
 r
2-oxoglutarate + NADH + H+ Escherichia coli
-
 (R)-2-hydroxyglutarate + NAD+
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography, hydroxyapatite column chromatography, and 5'-AMP-Sepharose affinity column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.27
-
 crude extract, at pH 7.5 and 37°C Escherichia coli
9.7
-
 after 3fold purification, at pH 7.5 and 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-hydroxyglutarate + NAD+
-
 Escherichia coli 2-oxoglutarate + NADH + H+
-
 r
2-oxoglutarate + NADH + H+
-
 Escherichia coli (R)-2-hydroxyglutarate + NAD+
-
 r

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
alphaKG reductase
-
 Escherichia coli
serA
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 40 the enzyme remains stable after 3 min incubation between 0 and 40°C, while 3 min at 45, 50 and 60°C leads to about 40, 70, and 100% loss of activity Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33.3
-
 2-oxoglutarate at pH 7.5 and 37°C Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0054
-
 L-serine at pH 7.5 and 37°C Escherichia coli
0.37
-
 D-2-hydroxyglutaric acid at pH 7.5 and 37°C Escherichia coli
12.7
-
 3-phosphoglycerate at pH 7.5 and 37°C Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0076
-
 at pH 7.5 and 37°C Escherichia coli L-serine

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
380
-
 2-oxoglutarate at pH 7.5 and 37°C Escherichia coli