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Literature summary for 1.1.1.39 extracted from

  • Babayev, H.; Mehvaliyeva, U.; Aliyeva, M.; Feyziyev, Y.; Guliyev, N.
    The study of NAD-malic enzyme in Amaranthus cruentus L. under drought (2014), Plant Physiol. Biochem., 81, 84-89.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
L-malate activates the enzyme in mitochondria Amaranthus cruentus
NAD+ activates the enzyme in mitochondria Amaranthus cruentus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Amaranthus cruentus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol in the pre-anthesis phase NAD-ME isoform with MW 115 kDa occurs in cytosol of mesophyll and bundle sheath cells of control and drought-exposed plants. In mesophyll cells 100% activity is found in cytosol fractions, and about 9.4% of the total NAD-ME activity is localized in cytosol of bundle sheath cells Amaranthus cruentus 5829
-
mitochondrion one of NAD-ME isoforms with MW 110 kDa is located in mitochondria of bundle sheath cells of control and drought-exposed plants, and another isoform of MW 121 kDa is formed in mitochondria of bundle sheath cells under influence of drought. After resuming watering the 121 kDa isoform disappears again. About 90.6% of the total NAD-ME activity is localized in mitochondrial stroma of bundle sheath cells Amaranthus cruentus 5739
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additional information several molecular forms of NAD-ME with different subcellular localization patterns in the studied phases of amaranth ontogenesis. No enzyme activity is observed experimentally in chloroplasts of mesophyll and bundle sheath cells Amaranthus cruentus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates the enzyme in mitochondria, kinetics, overview Amaranthus cruentus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
mitochondrial isozyme of bundle sheath cells of control and drought-exposed plants Amaranthus cruentus
115000
-
cytosolis isozyme of mesophyll and bundle sheath cells of control and drought-exposed plants Amaranthus cruentus
121000
-
mitochondrial isozyme of bundle sheath cells of drought-exposed plants Amaranthus cruentus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Amaranthus cruentus
-
pyruvate + CO2 + NADH
-
r

Organism

Organism UniProt Comment Textmining
Amaranthus cruentus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
bundle sheath cell
-
Amaranthus cruentus
-
leaf
-
Amaranthus cruentus
-
mesophyll cell in mesophyll cells, 100% activity is found in cytosol fractions Amaranthus cruentus
-
additional information no enzyme activity is observed experimentally in chloroplasts of mesophyll and bundle sheath cells Amaranthus cruentus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Amaranthus cruentus pyruvate + CO2 + NADH
-
r

Synonyms

Synonyms Comment Organism
NAD-malate dehydrogenase
-
Amaranthus cruentus
NAD-malic enzyme
-
Amaranthus cruentus
NAD-ME
-
Amaranthus cruentus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Amaranthus cruentus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 7.6 assay at Amaranthus cruentus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Amaranthus cruentus
NADH
-
Amaranthus cruentus

Expression

Organism Comment Expression
Amaranthus cruentus drought induces two of three isozymes in mesophyll and bundle sheath cells up