Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.39 extracted from

  • Niedzwiecka, N.; Skorkowski, E.F.
    Purification and properties of malic enzyme from herring Clupea harengus spermatozoa (2013), Comp. Biochem. Physiol. B, 164, 216-220.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Clarias gariepinus
ATP
-
Clupea harengus
ATP
-
Cyprinus carpio
ATP
-
Salmo salar
ATP
-
Salmo trutta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
NADH pH 7.0, 25°C Clupea harengus
0.16
-
(S)-malate pH 7.5, 25°C Clupea harengus
0.2
-
NAD+ pH 7.5, 25°C Clupea harengus
4.1
-
pyruvate pH 7.0, 25°C Clupea harengus
16.8
-
CO2 pH 7.0, 25°C Clupea harengus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates Cyprinus carpio
Mn2+ activates Salmo salar
Mn2+ activates Clarias gariepinus
Mn2+ activates Salmo trutta
Mn2+ activates, Km 0.08 mM in the decarboxylation/oxidation reaction Clupea harengus
Mn2+ activates, Km is 0.08 mM Clupea harengus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
4 * 61000, SDS-PAGE Clupea harengus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Cyprinus carpio
-
pyruvate + CO2 + NADH
-
r
(S)-malate + NAD+ Salmo salar
-
pyruvate + CO2 + NADH
-
r
(S)-malate + NAD+ Clarias gariepinus
-
pyruvate + CO2 + NADH
-
r
(S)-malate + NAD+ Clupea harengus
-
pyruvate + CO2 + NADH
-
r
(S)-malate + NAD+ Salmo trutta
-
pyruvate + CO2 + NADH
-
r

Organism

Organism UniProt Comment Textmining
Clarias gariepinus
-
-
-
Clupea harengus
-
-
-
Clupea harengus
-
from the Baltic Sea
-
Cyprinus carpio
-
-
-
Salmo salar
-
-
-
Salmo trutta
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 84.7fold by anion exchange and affinity chromatography, and gel filtration Clupea harengus
native NAD-preferring malic enzyme 84.7fold by anion exchange and affinity chromatography followed by gel filtration Clupea harengus

Source Tissue

Source Tissue Comment Organism Textmining
spermatozoon high exnzyme activity Clupea harengus
-
spermatozoon two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) Cyprinus carpio
-
spermatozoon two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) Salmo salar
-
spermatozoon two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) Clarias gariepinus
-
spermatozoon two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) Clupea harengus
-
spermatozoon two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) Salmo trutta
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.25
-
purified native enzyme, pH 7.0, 25°C, carboxylation reaction Clupea harengus
4.25
-
purified native enzyme, pH 7.0, 25°C, pyruvate carboxylation Clupea harengus
36.09
-
purified native enzyme, pH 7.5, 25°C, decarboxylation reaction Clupea harengus
36.09
-
purified native enzyme, pH 7.5, 25°C, malate decarboxylation Clupea harengus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Cyprinus carpio pyruvate + CO2 + NADH
-
r
(S)-malate + NAD+
-
Salmo salar pyruvate + CO2 + NADH
-
r
(S)-malate + NAD+
-
Clarias gariepinus pyruvate + CO2 + NADH
-
r
(S)-malate + NAD+
-
Clupea harengus pyruvate + CO2 + NADH
-
r
(S)-malate + NAD+
-
Salmo trutta pyruvate + CO2 + NADH
-
r
(S)-malate + NAD+ the decarboxylation reaction is preferred, overview Clupea harengus pyruvate + CO2 + NADH
-
r
pyruvate + CO2 + NADH the rate of carboxylation of pyruvate to malate is lower than for the decarboxylation reaction Clupea harengus (S)-malate + NAD+
-
r

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE Clupea harengus
tetramer 4 * 61000, SDS-PAGE Clupea harengus

Synonyms

Synonyms Comment Organism
NAD-preferring malic enzyme
-
Cyprinus carpio
NAD-preferring malic enzyme
-
Salmo salar
NAD-preferring malic enzyme
-
Clarias gariepinus
NAD-preferring malic enzyme
-
Clupea harengus
NAD-preferring malic enzyme
-
Salmo trutta
NAD-preferring ME
-
Clupea harengus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Cyprinus carpio
25
-
assay at Salmo salar
25
-
assay at Clarias gariepinus
25
-
assay at Clupea harengus
25
-
assay at Salmo trutta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
carboxylation reaction Clupea harengus
7
-
pyruvate carboxylation Clupea harengus
7.5
-
assay at Cyprinus carpio
7.5
-
assay at Salmo salar
7.5
-
assay at Clarias gariepinus
7.5
-
assay at Salmo trutta
7.5
-
decarboxylation reaction Clupea harengus
7.5
-
malate decarboxylation Clupea harengus

pH Range

pH Minimum pH Maximum Comment Organism
6 8 decarboxylation reaction, activity range Clupea harengus
6 9 activity range, profile overview Clupea harengus
6 7.5 carboxylation reaction, activity range Clupea harengus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Cyprinus carpio
NAD+
-
Salmo salar
NAD+
-
Clarias gariepinus
NAD+
-
Clupea harengus
NAD+
-
Salmo trutta
NAD+ dependent on Clupea harengus
NADH
-
Cyprinus carpio
NADH
-
Salmo salar
NADH
-
Clarias gariepinus
NADH
-
Clupea harengus
NADH
-
Salmo trutta

General Information

General Information Comment Organism
metabolism fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme Cyprinus carpio
metabolism fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme Salmo salar
metabolism fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme Clarias gariepinus
metabolism fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme Clupea harengus
metabolism fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme Salmo trutta