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Literature summary for 1.1.1.39 extracted from

  • Tronconi, M.A.; Fahnenstich, H.; Gerrard Weehler, M.C.; Andreo, C.S.; Fluegge, U.I.; Drincovich, M.F.; Maurino, V.G.
    Arabidopsis NAD-malic enzyme functions as a homodimer and heterodimer and has a major impact on nocturnal metabolism (2008), Plant Physiol., 146, 1540-1552.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene AtNAD-ME1, DNA and amino acid sequence determination and analysis, phylogenetic tree Arabidopsis thaliana
gene AtNAD-ME2, DNA and amino acid sequence determination and analysis, phylogenetic tree Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
NAD+ pH 7.4, 30°C, isozyme NAD-ME1 Arabidopsis thaliana
0.5
-
NAD+ pH 7.4, 30°C, isozyme NAD-ME2 Arabidopsis thaliana
3
-
(S)-malate pH 7.4, 30°C, isozyme NAD-ME1 Arabidopsis thaliana
3
-
(S)-malate pH 7.4, 30°C, isozyme NAD-ME2 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Arabidopsis thaliana 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
2 * 58000, beta-subunit, isozyme NAD-ME2, SDS-PAGE Arabidopsis thaliana
65000
-
2 * 65000, alpha-subunit, isozyme NAD-ME1, SDS-PAGE Arabidopsis thaliana
117500
-
gel filtration, isozyme NAD-ME2 Arabidopsis thaliana
120000
-
gel filtration, isozyme NAD-ME1 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Arabidopsis thaliana the enzyme plays a central role in the metabolite flux through the tricarboxylic acid cycle, overview pyruvate + CO2 + NADH
-
r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8L7K9 isozyme NAD-ME2; isozyme NAD-ME2 encoded by gene AtNAD-ME2
-
Arabidopsis thaliana Q9SIU0 isozyme NAD-ME1; isozyme NAD-ME1 encoded by gene AtNAD-ME1
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf leaf crude extracts contain about 20% higher NAD-ME specific activities at the end of the night period than at the end of the day period, isozyme NAD-ME1 is more abundant during the night period Arabidopsis thaliana
-
leaf leaf crude extracts contain about 20% higher NAD-ME specific activities at the end of the night period than at the end of the day period, isozyme NAD-ME2 is more abundant during the night period Arabidopsis thaliana
-
additional information tissue specific expression of the isozyme, overview Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Arabidopsis thaliana pyruvate + CO2 + NADH
-
r
(S)-malate + NAD+ the enzyme plays a central role in the metabolite flux through the tricarboxylic acid cycle, overview Arabidopsis thaliana pyruvate + CO2 + NADH
-
r

Subunits

Subunits Comment Organism
dimer 2 * 58000, beta-subunit, isozyme NAD-ME2, SDS-PAGE Arabidopsis thaliana
dimer 2 * 65000, alpha-subunit, isozyme NAD-ME1, SDS-PAGE Arabidopsis thaliana
More the isozyme NAD-ME1 is grouped into the clade that includes enzymes with alpha-subunits with molecular masses of approximately 65 kDa in the plant NAD-ME phylogenetic tree, isozymes NAD-ME1 and NAD-ME2 form both homo- and heterooligomers in vitro and in vivo, overview Arabidopsis thaliana
More the isozyme NAD-ME2 is grouped into the clades with enzymes possessing beta-subunits with molecular masses of approximately 58 kD in the plant NAD-ME phylogenetic tree, isozymes NAD-ME1 and NAD-ME2 form both homo- and heterooligomers in vitro and in vivo, overview Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
NAD-malic enzyme
-
Arabidopsis thaliana
NAD-ME1
-
Arabidopsis thaliana
NAD-ME2
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
31.1
-
(S)-malate pH 7.4, 30°C, isozyme NAD-ME1 Arabidopsis thaliana
44.1
-
(S)-malate pH 7.4, 30°C, isozyme NAD-ME2 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
Arabidopsis thaliana
6.6
-
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Arabidopsis thaliana
NADH
-
Arabidopsis thaliana