Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fumarate | activates in both reaction directions synergistically with L-malate both binding at separate allosteric sites different from the active site, R105 and K143 are involved | Ascaris suum | |
L-malate | activates in both reaction directions synergistically with fumarate both binding at separate allosteric sites different from the active site, R105 and K143 are involved | Ascaris suum |
Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes in Escherichia coli strain M15 | Ascaris suum |
Protein Variants | Comment | Organism |
---|---|---|
K143A | site-directed mutagenesis, malate binding residue, mutant shows highly increased kcat for malate and fumarate compared to the wild-type enzyme | Ascaris suum |
R105A | site-directed mutagenesis, fumarate binding residue, mutant shows 7-8fold reduced initial velocity with malate and Mg2+ compared to the wild-type enzyme, and is no longer activated by fumarate and malate | Ascaris suum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | inhibits the reductive carboxylation reaction, inhibitory effect is about 20fold reduced by binding of fumarate and L-malate | Ascaris suum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | detailed kinetic mechanism study, steady-state kinetics | Ascaris suum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Ascaris suum | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Ascaris suum | |
Mn2+ | required | Ascaris suum | |
additional information | the reaction is dependent on divalent metal ions | Ascaris suum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Ascaris suum | - |
pyruvate + CO2 + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ascaris suum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Ascaris suum | pyruvate + CO2 + NADH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NAD-malic enzyme | - |
Ascaris suum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Ascaris suum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Ascaris suum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Ascaris suum | |
NADH | - |
Ascaris suum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Ascaris suum |