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Literature summary for 1.1.1.39 extracted from

  • Wedding, R.T.; Black, M.K.
    Physical and kinetic properties and regulation of the NAD malic enzyme purified from leaves of Crassula argentea (1983), Plant Physiol., 72, 1021-1028.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.39

Activating Compound

Activating Compound Comment Organism Structure
1-N6-etheno-CoA activates Crassula argentea
AMP competitive activation Crassula argentea
coenzyme A activates Crassula argentea
fructose 1,6-bisphosphate activates Crassula argentea
fumarate fumarate2- is a strong activator Crassula argentea
phosphoenolpyruvate activates at low concentrations, decativation at high concentrations Crassula argentea

Inhibitors

Inhibitors Comment Organism Structure
citrate competitive Crassula argentea
Cl-
-
 Crassula argentea
NADH product inhibition Crassula argentea
phosphoenolpyruvate activates at low concentrations, deactivation at high concentrations Crassula argentea
pyruvate product inhibition Crassula argentea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 NADH activation by Mn2+ Crassula argentea
0.59
-
 (S)-malate activation by Mn2+ Crassula argentea
0.77
-
 NAD+ activation by Mg2+ Crassula argentea
0.8
-
 (S)-malate activation by Mn2+ Crassula argentea
6.03
-
 (S)-malate activation by Mg2+ Crassula argentea
13.48
-
 CO2 activation by Mg2+ Crassula argentea
15.03
-
 pyruvate activation by Mn2+ Crassula argentea

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ uses Mg2+ or Mn2+ as the required divalent cation Crassula argentea
Mn2+ divalent cation required, Mg2+ or Mn2+ Crassula argentea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
119000
-
 dimer, disc gel electrophoresis Crassula argentea
231000
-
 tetramer, disc gel electrophoresis Crassula argentea
350000
-
 non-denaturing PAGE Crassula argentea
491000
-
 tetramer, disc gel electrophoresis Crassula argentea

Organism

Organism UniProt Comment Textmining
Crassula argentea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Crassula argentea

Reaction

Reaction Comment Organism Reaction ID
(S)-malate + NAD+ = pyruvate + CO2 + NADH + H+ sequential mechanism Crassula argentea
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Crassula argentea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35.4
-
-
 Crassula argentea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+ 14% of the activity with NAD+ Crassula argentea pyruvate + CO2 + NADPH
-
 ?
malate + NAD+ activity of the reverse reaction is 1.5% of that of the forward reaction Crassula argentea pyruvate + CO2 + NADH + H+
-
 r
pyruvate + CO2 + NADH activity is 1.5% of the decarboxylation of (S)-malate Crassula argentea (S)-malate + NAD+
-
 r

Cofactor

Cofactor Comment Organism Structure
NAD+ cofactor Crassula argentea
NADP+ cofactor Crassula argentea
NADP+ 14% of the activity with NAD+ Crassula argentea