Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a C-terminally His6-tagged fusion protein | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
NADH | pH 7.5, 37°C | Bacillus subtilis | |
0.15 | - |
NAD+ | pH 7.5, 37°C | Bacillus subtilis | |
2.85 | - |
2,4,6/3,5-pentahydroxycyclohexanone | pH 7.5, 37°C | Bacillus subtilis | |
2.85 | - |
scyllo-inosose | pH 7.5, 37°C | Bacillus subtilis | |
17.1 | - |
scyllo-Inositol | pH 7.5, 37°C | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
scyllo-inositol + NAD+ | Bacillus subtilis | the enzyme is part of a scyllo-inositol degradation pathway leading to acetyl-CoA | 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ | - |
r | |
scyllo-inositol + NAD+ | Bacillus subtilis 168 | the enzyme is part of a scyllo-inositol degradation pathway leading to acetyl-CoA | 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P40332 | - |
- |
Bacillus subtilis 168 | P40332 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:scyllo-inositol-grown cell | - |
Bacillus subtilis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
scyllo-inositol + NAD+ | no activity with NADP+ | Bacillus subtilis | 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ | 2,4,6/3,5-pentahydroxycyclohexanone i.e. (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone i.e. scyllo-inosose | r | |
scyllo-inositol + NAD+ | the enzyme is part of a scyllo-inositol degradation pathway leading to acetyl-CoA | Bacillus subtilis | 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ | - |
r | |
scyllo-inositol + NAD+ | no activity with NADP+ | Bacillus subtilis 168 | 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ | 2,4,6/3,5-pentahydroxycyclohexanone i.e. (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone i.e. scyllo-inosose | r | |
scyllo-inositol + NAD+ | the enzyme is part of a scyllo-inositol degradation pathway leading to acetyl-CoA | Bacillus subtilis 168 | 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ | - |
r | |
scyllo-inositol + NAD+ | - |
Bacillus subtilis | scyllo-inosose + NADH + H+ | - |
r | |
scyllo-inositol + NAD+ | - |
Bacillus subtilis 168 | scyllo-inosose + NADH + H+ | - |
r | |
scyllo-inosose + NADH + H+ | - |
Bacillus subtilis | scyllo-inositol + NAD+ | - |
r | |
scyllo-inosose + NADH + H+ | - |
Bacillus subtilis 168 | scyllo-inositol + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
IolX | - |
Bacillus subtilis |
yisS | formeryl, gene name | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADP+ | Bacillus subtilis | |
NAD+ | - |
Bacillus subtilis | |
NAD+ | no activity with NADP+ | Bacillus subtilis | |
NADH | - |
Bacillus subtilis |
Organism | Comment | Expression |
---|---|---|
Bacillus subtilis | induced during growth on scyllo-inositol | up |
Bacillus subtilis | enzyme is prominently expressed when Bacillus subtilis is grown on medium containing scyllo-inositol | up |
General Information | Comment | Organism |
---|---|---|
malfunction | inactivation of iolX impairs growth with scyllo-inositol as the carbon source | Bacillus subtilis |
physiological function | the enzyme is part of a scyllo-inositol degradation pathway leading to acetyl-CoA | Bacillus subtilis |