Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-malate | 24.2% substrate inhibition at 20 mM | Zea mays | |
4-coumaric acid | 13.4% inhibition at 0.1 mM | Zea mays | |
ATP | 43.7% inhibition at 5 mM | Zea mays | |
Cu2+ | 25% inhibition at 0.1 mM | Zea mays | |
ferulic acid | 24.5% inhibition at 0.1 mM | Zea mays | |
oxaloacetate | 27.4% substrate inhibition at 1 mM | Zea mays | |
Zn2+ | 25% inhibition at 1 mM | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | bound | Zea mays | 5618 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
phosphate | 6.6% activation at 50 mM compared to Tris at 50 mM | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Zea mays | - |
oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | Zea mays VA35 | - |
oxaloacetate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Zea mays VA35 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | much greater activity of cell wall-associated malate dehydrogenase in the intensively growing lateral roots compared to primary root with decreased growth rates | Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Zea mays | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Zea mays VA35 | oxaloacetate + NADH + H+ | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
oxaloacetate reduction | Zea mays |
8.5 | - |
L-malate oxidation | Zea mays |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | oxaloacetate reduction, 50% of maximal activity at pH 5.5, maximal activity at pH 7.5 | Zea mays |
6 | 9 | malate oxidation, 10% of maximal activity at pH 6.0, maximal activity at pH 8.5 | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Zea mays | |
NADH | - |
Zea mays |
General Information | Comment | Organism |
---|---|---|
malfunction | exposure of maize plants to excess concentrations of Zn2+ and Cu2+ in the hydroponic solution inhibited lateral root growth, decreased malate dehydrogenase activity and changed isoform profiles | Zea mays |
additional information | a significant decrease of both mass and elongation of maize roots and shoots, as well as loss of root turgor and completely repressed lateral root growth were demonstrated in 0.1 mM Cu2+ and 5 mM Zn2+ treatments, overview | Zea mays |