Cloned (Comment) | Organism |
---|---|
from roots, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
NADH | pH 7.5, temperature not specified in the publication, native enzyme | Zea mays | |
0.029 | - |
NADH | pH 7.5, temperature not specified in the publication, recombinant enzyme | Zea mays | |
0.126 | - |
oxaloacetate | pH 7.5, temperature not specified in the publication, native enzyme | Zea mays | |
0.289 | - |
oxaloacetate | pH 7.5, temperature not specified in the publication, recombinant enzyme | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Zea mays | 5829 | - |
plasma membrane | associated | Spinacia oleracea | 5886 | - |
plasma membrane | associated | Zea mays | 5886 | - |
plasma membrane | associated | Brassica oleracea | 5886 | - |
soluble | - |
Zea mays | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, recombinant enzyme, SDS-PAGE, x * 35560, plasma membrane-associated enzyme, sequence calculation | Zea mays |
35560 | - |
x * 35000, recombinant enzyme, SDS-PAGE, x * 35560, plasma membrane-associated enzyme, sequence calculation | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Spinacia oleracea | - |
oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | Zea mays | - |
oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | Brassica oleracea | - |
oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | Zea mays Goldener Badischer Landmais | - |
oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH + H+ | Zea mays | - |
(S)-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | Zea mays Goldener Badischer Landmais | - |
(S)-malate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica oleracea | - |
two isozymes | - |
Spinacia oleracea | - |
- |
- |
Zea mays | - |
- |
- |
Zea mays Goldener Badischer Landmais | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from leaves bound to plasma membranes by affinity chromatography | Spinacia oleracea |
native enzyme from leaves, and two isozymes from roots bound to plasma membranes by affinity chromatography, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Zea mays |
native two isozymes from inflorescences bound to plasma membranes by affinity chromatography | Brassica oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
inflorescence | - |
Brassica oleracea | - |
leaf | - |
Spinacia oleracea | - |
leaf | PM-associated MDH and soluble MDH isozymes | Zea mays | - |
root | root isozymes | Zea mays | - |
seed | PM-associated MDH isozyme | Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Spinacia oleracea | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Zea mays | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Brassica oleracea | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Zea mays Goldener Badischer Landmais | oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Zea mays | (S)-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Zea mays Goldener Badischer Landmais | (S)-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, recombinant enzyme, SDS-PAGE, x * 35560, plasma membrane-associated enzyme, sequence calculation | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
recombinant enzyme | Zea mays |
7.5 | - |
assay at | Spinacia oleracea |
7.5 | - |
assay at | Brassica oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Spinacia oleracea | |
NAD+ | - |
Zea mays | |
NAD+ | - |
Brassica oleracea | |
NADH | - |
Spinacia oleracea | |
NADH | - |
Zea mays | |
NADH | - |
Brassica oleracea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Zea mays | plasma membrane-associated enzyme, sequence calculation | - |
5.75 |
Zea mays | purified recombinant enzyme, isoelectric focussing | - |
6.8 |
General Information | Comment | Organism |
---|---|---|
evolution | the plasma membrane-associated isozyme belongs belongs to the lactate dehydrogenase/MDH superfamily, MDH type 2 family | Zea mays |
physiological function | besides its function in malate synthesis, MDH is responsible for the exchange of reducing equivalents between metabolic pathways in distinct cell compartments | Spinacia oleracea |
physiological function | besides its function in malate synthesis, MDH is responsible for the exchange of reducing equivalents between metabolic pathways in distinct cell compartments | Zea mays |
physiological function | besides its function in malate synthesis, MDH is responsible for the exchange of reducing equivalents between metabolic pathways in distinct cell compartments | Brassica oleracea |